Catalases and thioredoxin peroxidase protect Saccharomyces cerevisiae against Ca2+-induced mitochondrial membrane permeabilization and cell death

dc.creatorKowaltowski, AJ
dc.creatorVercesi, AE
dc.creatorRhee, SG
dc.creatorNetto, LES
dc.date2000
dc.dateMAY 12
dc.date2014-12-02T16:26:19Z
dc.date2015-11-26T16:26:48Z
dc.date2014-12-02T16:26:19Z
dc.date2015-11-26T16:26:48Z
dc.date.accessioned2018-03-28T23:07:39Z
dc.date.available2018-03-28T23:07:39Z
dc.identifierFebs Letters. Elsevier Science Bv, v. 473, n. 2, n. 177, n. 182, 2000.
dc.identifier0014-5793
dc.identifierWOS:000087126800013
dc.identifier10.1016/S0014-5793(00)01526-X
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/78495
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/78495
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/78495
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1268999
dc.descriptionThe involvement of reactive oxygen species in Ca2+-induced mitochondrial membrane permeabilization and cell viability was studied using yeast cells in which the thioredoxin peroxidase (TPx) gene was disrupted and/or catalase was inhibited by 3-amino-1,2,4-triazole (ATZ) treatment. Wild-type Saccharomyces cerevisiae cells were very resistant to Ca2+ and inorganic phosphate or t-butyl hydroperoxide-induced mitochondrial membrane permeabilization, but suffered an immediate decrease in mitochondrial membrane potential when treated with Ca2+ and the dithiol binding reagent phenylarsine oxide. In contrast, S, cerevisiae spheroblasts lacking the TPx gene and/or treated with ATZ suffered a decrease in mitochondrial membrane potential, generated higher amounts of hydrogen peroxide and had decreased viability under these conditions. In all cases, the decrease in mitochondrial membrane potential could be inhibited by ethylene glycol-bis(beta-aminoethyl ether) N,N,N',N'-tetraacetic acid, dithiothreitol or ADP, but not by cyclosporin A. We conclude that TPx and catalase act together, maintaining cell viability and protecting S, cerevisiae mitochondria against Ca2+-promoted membrane permeabilization, which presents similar characteristics to mammalian permeability transition. (C) 2000 Federation of European Biochemical Societies.
dc.description473
dc.description2
dc.description177
dc.description182
dc.languageen
dc.publisherElsevier Science Bv
dc.publisherAmsterdam
dc.publisherHolanda
dc.relationFebs Letters
dc.relationFEBS Lett.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectmitochondrion
dc.subjectyeast
dc.subjectantioxidant
dc.subjectmitochondriai permeability transition
dc.subjectcell death
dc.subjectCytochrome-c
dc.subjectYeast Mitochondria
dc.subjectOxidative Stress
dc.subjectTransition Pore
dc.subjectIn-situ
dc.subjectHydrogen-peroxide
dc.subjectApoptosis
dc.subjectBcl-2
dc.subjectAntioxidant
dc.subjectCalcium
dc.titleCatalases and thioredoxin peroxidase protect Saccharomyces cerevisiae against Ca2+-induced mitochondrial membrane permeabilization and cell death
dc.typeArtículos de revistas


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