SCREENING OF MICROBIAL LIPASES AND EVALUATION OF THEIR POTENTIAL TO PRODUCE GLYCERIDES WITH HIGH GAMMA LINOLENIC ACID CONCENTRATION

dc.creatorFregolente, PBL
dc.creatorFregolente, LV
dc.creatorMaciel, MRW
dc.creatorCarvalho, PO
dc.date2009
dc.dateOCT-DEC
dc.date2014-07-30T18:35:44Z
dc.date2015-11-26T16:25:26Z
dc.date2014-07-30T18:35:44Z
dc.date2015-11-26T16:25:26Z
dc.date.accessioned2018-03-28T23:06:12Z
dc.date.available2018-03-28T23:06:12Z
dc.identifierBrazilian Journal Of Microbiology. Soc Brasileira Microbiologia, v. 40, n. 4, n. 747, n. 756, 2009.
dc.identifier1517-8382
dc.identifierWOS:000270684300004
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/71560
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/71560
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1268629
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionGamma-linolenic acid (GLA, 18: 3, cis-6,9,12-octadecatrienoic acid), an important compound in n-6 eicosanoid family biosynthesis, occurs in the lipids of a few plant and microbial sources. This study focused on the screening of microbial strains with suitable lipase activity for enrichment of GLA by selective hydrolysis of the borage oil (21.6 % of GLA/total fatty acids). Firstly, 352 microrganisms were tested for their lipolytic capacity using screening techniques on agar plates containing borage oil, strains were then selected and screened for their activity (U/mg) using both submerged fermentation (SmF) and solid state fermentation (SSF). The rate of hydrolysis and the selective preference of these hydrolytic enzymes towards fatty acids, with a special focus on enrichment of GLA were studied and compared with those obtained by two commercially-available lipases. Only one of the lipases tested during this study displayed selectivity, discriminating the GLA during the hydrolysis reaction. Using the enzymatic extract from Geotrichum candidum as a biocatalyst of the reaction, it was possible to obtain a percentage of 41.7% of GLA in acylglycerols fraction when the borage oil was treated in a fixed-bed reactor for 24 hours at 30 degrees C.
dc.description40
dc.description4
dc.description747
dc.description756
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageen
dc.publisherSoc Brasileira Microbiologia
dc.publisherSao Paulo
dc.publisherBrasil
dc.relationBrazilian Journal Of Microbiology
dc.relationBraz. J. Microbiol.
dc.rightsaberto
dc.sourceWeb of Science
dc.subjectgamma linolenic acid
dc.subjectborage oil
dc.subjectlipase
dc.subjectGeotrichum candidum
dc.subjectCandida-cylindracea Lipase
dc.subjectPolyunsaturated Fatty-acids
dc.subjectEvening Primrose Oil
dc.subjectDocosahexaenoic Acid
dc.subjectCatalyzed Hydrolysis
dc.subjectSelective Hydrolysis
dc.subjectFusarium-oxysporum
dc.subjectGeotrichum Sp
dc.subjectBorage Oil
dc.subjectEnrichment
dc.titleSCREENING OF MICROBIAL LIPASES AND EVALUATION OF THEIR POTENTIAL TO PRODUCE GLYCERIDES WITH HIGH GAMMA LINOLENIC ACID CONCENTRATION
dc.typeArtículos de revistas


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