Assessment of the diagnostic potential of Immmunocapture-PCR and Immuno-PCR for Citrus Variegated Chlorosis

dc.creatorPeroni, LA
dc.creatordos Reis, JRR
dc.creatorDella Coletta, H
dc.creatorde Souza, AA
dc.creatorMachado, MA
dc.creatorStach-Machado, DR
dc.date2008
dc.dateOCT
dc.date2014-11-16T21:21:51Z
dc.date2015-11-26T16:25:21Z
dc.date2014-11-16T21:21:51Z
dc.date2015-11-26T16:25:21Z
dc.date.accessioned2018-03-28T23:06:08Z
dc.date.available2018-03-28T23:06:08Z
dc.identifierJournal Of Microbiological Methods. Elsevier Science Bv, v. 75, n. 2, n. 302, n. 307, 2008.
dc.identifier0167-7012
dc.identifierWOS:000259942500023
dc.identifier10.1016/j.mimet.2008.06.024
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/55010
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/55010
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/55010
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1268613
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionXylella fastidiosa causes significant losses in many economically important crops. An efficient pathogen detection system is critical for epidemiology studies, particularly when large sample size is involved. In this study we report the development of immunomolecular assays like Immmunocapture-PCR and Immuno-PCR for direct detection of X fastidiosa without DNA isolation. Whereas the reactivity of ELISA and PCR ranged from 10(6) to 10(4) bacterial cells, the IC-PCR sensitivity was up to 10(3) and the detection limit of I-PCR was up to 10(1) bacterial cells. These methods can use either plant sample extracts or cultivated media. and show no cross reaction for any other endophytic citrus-bacteria. Therefore, IC-PCR and I-PCR assays provide an alternative for quick and very sensitive methods to screening X fastidiosa, with the advantage of not requiring any concentration or DNA purification steps while still allowing an accurate diagnosis of CVC. (C) 2008 Elsevier B.V. All rights reserved.
dc.description75
dc.description2
dc.description302
dc.description307
dc.descriptionFINEP (Financiadora de Estudos e Projetos)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.languageen
dc.publisherElsevier Science Bv
dc.publisherAmsterdam
dc.publisherHolanda
dc.relationJournal Of Microbiological Methods
dc.relationJ. Microbiol. Methods
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectXylella fastidiosa
dc.subjectCitrus Variegated Chlorosis
dc.subjectImmunocapture-PCR
dc.subjectImmuno-PCR
dc.subjectELISA
dc.subjectPCR
dc.subjectPolymerase-chain-reaction
dc.subjectNeurotoxin Type-a
dc.subjectXylella-fastidiosa
dc.subjectReaction Assay
dc.subjectSweet Orange
dc.subjectBacteria
dc.subjectDisease
dc.subjectToxin
dc.subjectIdentification
dc.subjectAmplification
dc.titleAssessment of the diagnostic potential of Immmunocapture-PCR and Immuno-PCR for Citrus Variegated Chlorosis
dc.typeArtículos de revistas


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