Purification and biological effects of C-type lectin isolated from Bothrops insularis venom

dc.creatorBraga, MDM
dc.creatorMartins, AMC
dc.creatorAmora, DN
dc.creatorde Menezes, DB
dc.creatorToyama, MH
dc.creatorToyama, DO
dc.creatorMarangoni, S
dc.creatorBarbosa, PSF
dc.creatorAlves, RD
dc.creatorFonteles, MC
dc.creatorMonteiro, HSA
dc.date2006
dc.date42156
dc.date2014-11-16T19:30:00Z
dc.date2015-11-26T16:24:57Z
dc.date2014-11-16T19:30:00Z
dc.date2015-11-26T16:24:57Z
dc.date.accessioned2018-03-28T23:05:46Z
dc.date.available2018-03-28T23:05:46Z
dc.identifierToxicon. Pergamon-elsevier Science Ltd, v. 47, n. 8, n. 859, n. 867, 2006.
dc.identifier0041-0101
dc.identifierWOS:000238962800005
dc.identifier10.1016/j.toxicon.2006.02.016
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/58440
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/58440
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/58440
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1268526
dc.descriptionBothrops insularis is a snake from Queimada Grande Island, which is an island located about 20 miles away from the southeastern coast of Brazil. Compared to other Brazilian species of Bothrops, the toxinology of B. insularis is still poorly understood. Its C-type lectin is involved in several biological processes including anticoagulant and platelet-modulating activities. We purified the C-type lectin (BiLec) from Bothrops insularis venom and investigated its effect in the isolated kidney. BiLec was purified after two chromatographic steps; firstly, the whole venom was submitted to an HPLC molecular exclusion chromatography followed by a second purification through affinity chromatography. B. insularis lectin (BiLec) was studied as to its effect on the renal function of isolated perfused rat kidneys with the use of six Wistar rats. The concentration of 10 mu g/mL increased perfusion pressure (PP; control(60) = 108.27 +/- 4.9; BiLec(60) = 112.9 +/- 5.4 mmHg; *p < 0.05) and renal vascular resistance (RVR; control(60)=5.38 +/- 0.51; BiLec(60)=6.01 +/- 0.57 mmHg; *p < 0.05). The urinary flow reduced significantly at 90 and 120min of perfusion (UF; control(120)= 0.160 +/- 0.020; BiLec(120) =0.082 +/- 0.008mL g(-1) min(-1); *p < 0.05). Glomerular filtration rate (GFR; control(120)=0.697 +/- 0.084; BiLec(120)=0.394 +/- 0.063 mL g(-1) min(-1); *p < 0.05) diminished only at 120 min. BiLec did not change the percentage of sodium (TNa+), potassium (TK+) and chloride tubular transport (TCl-). The histological alterations probably reflected direct injury on glomerular and tubular renal cells, as demonstrated by the rise in permeability of glomerular endothelial cells, revealed by the presence of a proteinaccous material in the Bowman space. We postulate that the C-type lectin B. insularis promoted its effects probably through interactions with endothelial cells or through the release of other mediators by tubular, mesangial and endothelial cells. (c) 2006 Elsevier Ltd. All rights reserved.
dc.description47
dc.description8
dc.description859
dc.description867
dc.languageen
dc.publisherPergamon-elsevier Science Ltd
dc.publisherOxford
dc.publisherInglaterra
dc.relationToxicon
dc.relationToxicon
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectBothrops insularis
dc.subjectlectin
dc.subjectbiological activity
dc.subjectrenal activity
dc.subjectJararacussu Snake-venom
dc.subjectBinding Lectin
dc.subjectLachesis-muta
dc.subjectPit Viper
dc.subjectProtein
dc.subjectExpression
dc.subjectCloning
dc.subjectKidney
dc.subjectGrowth
dc.subjectBjcul
dc.titlePurification and biological effects of C-type lectin isolated from Bothrops insularis venom
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución