Epithelial-stromal transition of MMP-7 immunolocalization in the rat ventral prostate following bilateral orchiectorny

dc.creatorFelisbino, SL
dc.creatorJustulin, LA
dc.creatorCarvalho, HF
dc.creatorTaboga, SR
dc.date2007
dc.dateOCT
dc.date2014-11-16T17:31:09Z
dc.date2015-11-26T16:24:17Z
dc.date2014-11-16T17:31:09Z
dc.date2015-11-26T16:24:17Z
dc.date.accessioned2018-03-28T23:05:15Z
dc.date.available2018-03-28T23:05:15Z
dc.identifierCell Biology International. Academic Press Ltd Elsevier Science Ltd, v. 31, n. 10, n. 1173, n. 1178, 2007.
dc.identifier1065-6995
dc.identifierWOS:000248408800011
dc.identifier10.1016/j.cellbi.2007.03.034
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/65505
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/65505
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/65505
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1268402
dc.descriptionEpithelial cells from involuting rat ventral prostate (VP) express Matrilysin (MMP-7) mRNA. Herein, we investigated by immunohistochemistry the NIMP-7 protein location and its association with tissue changes following castration in the VP. Normal and castrated adult male Wistar rats were sacrificed at different times after surgery. VP was examined by immunocytochemistry and immunoprecipitation. Castration promoted a shrinking of prostate ducts with an extensive stromal remodeling. In the VP from normal rats, MMP-7 immunoreactivity was found in epithelial secretory granules. Three days after castration, immunostaining for MMP-7 was found in both the epithelial secretory granules and in the stroma just below the epithelium, mainly at the distal ductal tips. At seven and 21 days after castration, the immunostaining for MMP-7 was found only in the stromal space. Immunoprecipitation confirmed the specificity of the primary antibody by rescuing a pro-enzyme form (28 kDa) in the prostate extracts. The present results suggest that MMP-7 participates in the epithelial-stromal interface remodeling of the ventral prostate during the involution achieved by castration, probably in the degradation of components of the epithelial basement membrane. (c) 2007 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.
dc.description31
dc.description10
dc.description1173
dc.description1178
dc.languageen
dc.publisherAcademic Press Ltd Elsevier Science Ltd
dc.publisherLondon
dc.publisherInglaterra
dc.relationCell Biology International
dc.relationCell Biol. Int.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectmetalloproteinases
dc.subjectbasement membrane
dc.subjectcastration
dc.subjectmatrilysin
dc.subjectcollagen fibers
dc.subjectstroma
dc.subjectMatrix Metalloproteinases
dc.subjectDuctal System
dc.subjectMatrilysin Expression
dc.subjectRegional-variation
dc.subjectCastrated Rats
dc.subjectCell-death
dc.subjectActivation
dc.subjectInhibitors
dc.subjectRelease
dc.subjectLobe
dc.titleEpithelial-stromal transition of MMP-7 immunolocalization in the rat ventral prostate following bilateral orchiectorny
dc.typeArtículos de revistas


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