Determination of Crotalus durissus cascavella venom components that induce renal toxicity in isolated rat kidneys

dc.creatorMartins, AMC
dc.creatorToyama, MH
dc.creatorHavt, A
dc.creatorNovello, JC
dc.creatorMarangoni, S
dc.creatorFonteles, MC
dc.creatorMonteiro, HSA
dc.date2002
dc.dateAUG
dc.date2014-11-16T17:23:11Z
dc.date2015-11-26T16:24:17Z
dc.date2014-11-16T17:23:11Z
dc.date2015-11-26T16:24:17Z
dc.date.accessioned2018-03-28T23:05:15Z
dc.date.available2018-03-28T23:05:15Z
dc.identifierToxicon. Pergamon-elsevier Science Ltd, v. 40, n. 8, n. 1165, n. 1171, 2002.
dc.identifier0041-0101
dc.identifierWOS:000177655500012
dc.identifier10.1016/S0041-0101(02)00119-8
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/61368
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/61368
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/61368
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1268401
dc.descriptionEnvenomation by Crotalus durissus terrificus leads to coagulation disorders, myotoxicity, neurotoxicity and acute renal failure (ARF). The most serious systemic change and primary cause of death is ARF In this work, we used RP-HPLC to isolate crotoxin, convulxin and gyroxin from venom of the related subspecies Crotalus durissus cascavella and investigated the effects of these toxins on renal function in the isolated rat kidneys perfused with Krebs-Henseleit solution containing 6% of bovine serum albumin. The parameters studied included perfusion pressure (PP), renal vascular resistance (RVR), glomerular filtration rate (GFR), urinary flow (UF), percent of sodium tubular transport (%TNa+), percent of potassium tubular transport (%TK+) and percent of chloride tubular transport (%TCl-). Crotoxin (5 mug/ml) increased the PP, RVR, GFR, UF and decreased %TNa+, %TK+ and %TCl-, with gyroxin (5 mug/ml) the GFR remained stable during the 120 min of perfusion, whereas PP and RVR increased significantly and the %TNa+, %TK+ and %TCl- decreased significantly. Convulxin (5 mug/ml) had no effect on renal function. Crotoxin caused alterations in all renal parameters. Gyroxin produced a minor effect compared to crotoxin. These results indicated that crotoxin is the main componenet responsible for acute nephrotoxicity caused by C. d. cascavella venom. (C) 2002 Elsevier Science Ltd. All rights reserved.
dc.description40
dc.description8
dc.description1165
dc.description1171
dc.languageen
dc.publisherPergamon-elsevier Science Ltd
dc.publisherOxford
dc.publisherInglaterra
dc.relationToxicon
dc.relationToxicon
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectC.d. cascavella
dc.subjectcrotoxin
dc.subjectgyroxin
dc.subjectrenal perfusion system
dc.subjectPlatelet-aggregating Protein
dc.subjectTerrificus Venom
dc.subjectConvulxin
dc.subjectCrotoxin
dc.subjectActivation
dc.subjectCrotamine
dc.subjectCollagen
dc.subjectGyroxin
dc.subjectToxin
dc.titleDetermination of Crotalus durissus cascavella venom components that induce renal toxicity in isolated rat kidneys
dc.typeArtículos de revistas


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