Proliferation of fibroblasts cultured from normal gingiva and hereditary gingival fibromatosis is dependent on fatty acid synthase activity

dc.creatorAlmeida, JP
dc.creatorColetta, RD
dc.creatorSilva, SD
dc.creatorAgostini, M
dc.creatorVargas, PA
dc.creatorBozzo, L
dc.creatorGraner, E
dc.date2005
dc.dateFEB
dc.date2014-11-16T15:25:50Z
dc.date2015-11-26T16:23:45Z
dc.date2014-11-16T15:25:50Z
dc.date2015-11-26T16:23:45Z
dc.date.accessioned2018-03-28T23:04:50Z
dc.date.available2018-03-28T23:04:50Z
dc.identifierJournal Of Periodontology. Amer Acad Periodontology, v. 76, n. 2, n. 272, n. 278, 2005.
dc.identifier0022-3492
dc.identifierWOS:000228085500017
dc.identifier10.1902/jop.2005.76.2.272
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/70511
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/70511
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/70511
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1268300
dc.descriptionBackground: Fatty acid synthase (FAS) is the enzyme that synthesizes palmitate from malonyl-CoA and acetyl-CoA. Recent studies have shown that FAS is overexpressed in human cancers and that its activity is necessary for cell proliferation. Hereditary gingival fibromatosis (HGF) is a genetic disease manifested as a progressive enlargement of the gingiva. The pathogenesis of this condition is not understood; however, a proliferative advantage of HGF fibroblasts in comparison with cells from normal gingiva (NG) has been described. The aim of this study was to investigate the role of FAS in NG and HGF fibroblast proliferation. Methods: NG and HGF fibroblasts had their proliferative potential assessed by automated cell counting and immunocytochemistry against Ki-67 or proliferating cell nuclear antigen (PCNA). The production of FAS, androgen receptor (AR), and ErbB2 was analyzed by Western blot and the pattern of FAS expression studied by immunocytochemistry. FAS activity was blocked by the specific inhibitor cerulenin. Results: Higher proliferation rates were found in fibroblasts isolated from HGF than from NG. HGF fibroblasts with greater proliferative potential produced more FAS and AR than the cell lines with lower growth rates, and all studied cell lines produced similar amounts of the ErbB2 protein. In addition, the FAS inhibitor cerulenin was able to significantly reduce the proliferation of both NG and HGF cells. Conclusions: These results show that FAS is expressed by gingival fibroblasts and that highly proliferative HGF cells produced more FAS and AR than the other fibroblast cell lines. Moreover, FAS inhibition significantly reduced both NG and HGF fibroblast growth, suggesting a role for the androgen-driven fatty acid biosynthesis in their proliferation.
dc.description76
dc.description2
dc.description272
dc.description278
dc.languageen
dc.publisherAmer Acad Periodontology
dc.publisherChicago
dc.publisherEUA
dc.relationJournal Of Periodontology
dc.relationJ. Periodont.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectfatty acid synthase
dc.subjectfibroblasts/anatomy and physiology
dc.subjectfibromatosis
dc.subjectgingival
dc.subjectgingival anatomy and physiology
dc.subjectgingival hyperplasia
dc.subjectproteins, ErbB2
dc.subjectreceptors, androgen
dc.subjectSquamous-cell Carcinoma
dc.subjectProstate-cancer
dc.subjectGene-expression
dc.subjectTransforming Growth-factor-beta-1
dc.subjectProgression
dc.subjectBinding
dc.subjectMetabolism
dc.subjectInhibition
dc.subjectPathway
dc.subjectLine
dc.titleProliferation of fibroblasts cultured from normal gingiva and hereditary gingival fibromatosis is dependent on fatty acid synthase activity
dc.typeArtículos de revistas


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