Sperm retrieval techniques in rats with suppressed spermatogenesis by experimental cryptorchidism

dc.creatorRossi, LM
dc.creatorPereira, LAV
dc.creatorde Santis, L
dc.creatorPasqualotto, FF
dc.creatorIaconelli, A
dc.creatorOrtiz, V
dc.creatorBorges, E
dc.date2005
dc.dateFEB
dc.date2014-11-16T14:46:58Z
dc.date2015-11-26T16:23:29Z
dc.date2014-11-16T14:46:58Z
dc.date2015-11-26T16:23:29Z
dc.date.accessioned2018-03-28T23:04:42Z
dc.date.available2018-03-28T23:04:42Z
dc.identifierHuman Reproduction. Oxford Univ Press, v. 20, n. 2, n. 443, n. 447, 2005.
dc.identifier0268-1161
dc.identifierWOS:000226611300019
dc.identifier10.1093/humrep/deh549
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/72140
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/72140
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/72140
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1268264
dc.descriptionObjective: Our aim was to assess the suppression of spermatogenesis and sperm retrieval rate after testicular sperm extraction (TESE) or testicular sperm aspiration (TESA) in adult rats with surgically induced cryptorchidism. Methods: Adult rats were submitted to TESE and TESA procedures after 15 days of induced cryptorchidism. After spermatozoa retrieval, the testicles were extracted, weighed and a morphological analysis by conventional light microscopy was done. The numbers of spermatozoa retrieved in both TESA and TESE were rated and compared. Results: Histological analysis of the testicles revealed Sertoli cell-only syndrome in 60% of the testicles, and maturation arrest in the remaining cryptorchid testicles. Significant differences were seen in the number of spermatozoa retrieved (P < 0.05) between cryptorchidic and control rats. When sperm retrieval techniques were compared, no differences were detected in the number of spermatozoa obtained (P > 0.05). Conclusions: It seems that a 15 day period of cryptorchidism is enough to induce spermatogenesis disorders. No differences were detected in the number of spermatozoa retrieved in the right or left testicles, irrespective of the testicular pole. Furthermore, and even more importantly, no differences in the retrieval rate were seen between the two techniques.
dc.description20
dc.description2
dc.description443
dc.description447
dc.languageen
dc.publisherOxford Univ Press
dc.publisherOxford
dc.publisherInglaterra
dc.relationHuman Reproduction
dc.relationHum. Reprod.
dc.rightsfechado
dc.rightshttp://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html
dc.sourceWeb of Science
dc.subjectaspiration
dc.subjectcryptorchidism
dc.subjectextraction
dc.subjectsperm
dc.subjecttesticle
dc.subjectFine-needle-aspiration
dc.subjectUnilateral Undescended Testis
dc.subjectIn-vitro Fertilization
dc.subjectNonobstructive Azoospermia
dc.subjectOpen Biopsy
dc.subjectIntracytoplasmic Injection
dc.subjectTesticular Spermatozoa
dc.subjectImplantation Rates
dc.subjectMen
dc.subjectExtraction
dc.titleSperm retrieval techniques in rats with suppressed spermatogenesis by experimental cryptorchidism
dc.typeArtículos de revistas


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