Proteoglycan synthesis by human corneal explants submitted to laser in situ keratomileusis (LASIK)

dc.creatorMartins, SAR
dc.creatorCampos, MQ
dc.creatorVidal, BC
dc.creatorBerto, AGA
dc.creatorAguiar, JAK
dc.creatorMichelacci, YM
dc.date2007
dc.dateFEB 1
dc.date2014-11-16T13:25:04Z
dc.date2015-11-26T16:23:02Z
dc.date2014-11-16T13:25:04Z
dc.date2015-11-26T16:23:02Z
dc.date.accessioned2018-03-28T23:04:25Z
dc.date.available2018-03-28T23:04:25Z
dc.identifierMolecular Vision. Molecular Vision, v. 13, n. 17, n. 142, n. 150, 2007.
dc.identifier1090-0535
dc.identifierWOS:000244101600001
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/58337
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/58337
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/58337
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1268199
dc.descriptionPURPOSE: To evaluate the acute effects of laser in situ keratomileusis (LASIK) upon the synthesis of proteoglycans (PGs) and collagen fibril organization in human corneal explants. METHODS: Human corneas that had been rejected for transplants were obtained at Banco de Olhos of Hospital So Paulo. For each eye pair, one cornea was submitted to refractive surgery, and the other was used as its matched control. After surgery, the corneas were excised from the eyes and immediately placed in a Ham F-12 nutrient mixture containing S-35-sulfate for the metabolic labeling of PGs. After 24 h incubation, PGs were extracted and identified by a combination of agarose gel electrophoresis and enzymatic degradation with protease and specific glycosaminoglycan lyases. Histopathological and birefringence analysis were performed in fixed tissue slices. RESULTS: A marked decrease in S-35-sulfate incorporation in PGs was observed in corneal explants that received LASIK, especially concerning dermatan sulfate-PGs, with keratan sulfate- and heparan sulfate-PG synthesis reduced to a lower degree. Only low molecular weight PGs were present in the corneas, both before and 24 h after LASIK. No sign of wound healing processes were observed, but a marked change in corneal birefringence was seen following LASIK treatment. CONCLUSIONS: Laser application led to decreased PG biosynthesis in human corneal explants, with marked changes in the collagen fibril organization, as revealed by changes in the tissue birefringence.
dc.description13
dc.description17
dc.description142
dc.description150
dc.languageen
dc.publisherMolecular Vision
dc.publisherAtlanta
dc.publisherEUA
dc.relationMolecular Vision
dc.relationMol. Vis.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectKeratan Sulfate Proteoglycan
dc.subjectPhotorefractive Keratectomy
dc.subjectFlavobacterium-heparinum
dc.subjectEpithelial Debridement
dc.subjectChondroitin Sulfate
dc.subjectBasement-membrane
dc.subjectCore Proteins
dc.subjectLumican
dc.subjectRabbits
dc.subjectLight
dc.titleProteoglycan synthesis by human corneal explants submitted to laser in situ keratomileusis (LASIK)
dc.typeArtículos de revistas


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