Microbiological comparative study of isolates of Edwardsiella tarda isolated in different countries from fish and humans

dc.creatorNucci, C
dc.creatorda Silveira, WD
dc.creatorCorrea, SD
dc.creatorNakazato, G
dc.creatorBando, SY
dc.creatorRibeiro, MA
dc.creatorde Castro, AFP
dc.date2002
dc.dateOCT 2
dc.date2014-11-17T02:40:34Z
dc.date2015-11-26T16:22:46Z
dc.date2014-11-17T02:40:34Z
dc.date2015-11-26T16:22:46Z
dc.date.accessioned2018-03-28T23:04:13Z
dc.date.available2018-03-28T23:04:13Z
dc.identifierVeterinary Microbiology. Elsevier Science Bv, v. 89, n. 1, n. 29, n. 39, 2002.
dc.identifier0378-1135
dc.identifierWOS:000178224500003
dc.identifier10.1016/S0378-1135(02)00151-7
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/56687
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/56687
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/56687
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1268150
dc.descriptionIt is difficult to use tissue culture assays to investigate adherence and other properties of Edwardsiella tarda because the organism is invasive and produces a potent hemolysin. We therefore relied on polymerase chain reaction (PCR) to determine the occurrence of genes for enterotoxins (LT-I, EAST-1), Shiga toxin (Stx-1, Stx-2), cytotoxic necrotizing factors (CNF-1, CNF-2), aerobactin, invasion plasmid of enteroinvasive Escherichia coli, EPEC adherence factor (EAF), intimin (Eae), enterohemolysin (EntHly) and hemolysin (lily) in 53 isolates of E. tarda from humans and fish from several countries. All isolates were negative for all genes investigated by PCR. Adhesion to and invasion of HeLa cells were determined by using the unusually short incubation time of 1 h or 30 min. All isolates adhered and invaded in these tests. Finally, a random amplified polymorphic DNA (RAPD) test distinguished, with a few exceptions, isolates of human and fish origin. (C) 2002 Elsevier Science B.V. All rights reserved.
dc.description89
dc.description1
dc.description29
dc.description39
dc.languageen
dc.publisherElsevier Science Bv
dc.publisherAmsterdam
dc.publisherHolanda
dc.relationVeterinary Microbiology
dc.relationVet. Microbiol.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectEdwardsiella tarda
dc.subjecthuman and fish isolates
dc.subjectzoonoses
dc.subjectPCR
dc.subjectadhesion to HeLa
dc.subjectRAPD
dc.subjectEnterotoxigenic Escherichia-coli
dc.subjectPolymerase Chain-reaction
dc.subjectShigella-flexneri
dc.subjectHep-2 Cells
dc.subjectHela-cells
dc.subjectVirulence
dc.subjectAdherence
dc.subjectStrains
dc.subjectSequence
dc.subjectInvasion
dc.titleMicrobiological comparative study of isolates of Edwardsiella tarda isolated in different countries from fish and humans
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución