Ultrastructural, immunohistochemical and biochemical analysis of glycosarninoglycans and proteoglycans in the mouse, pubic symphysis during pregnancy

dc.creatorPinheiro, MC
dc.creatorMora, OA
dc.creatorCaldini, EG
dc.creatorBattlehner, CN
dc.creatorJoazeiro, PP
dc.creatorToledo, OMS
dc.date2005
dc.dateJUN
dc.date2014-11-16T10:29:59Z
dc.date2015-11-26T16:22:07Z
dc.date2014-11-16T10:29:59Z
dc.date2015-11-26T16:22:07Z
dc.date.accessioned2018-03-28T23:03:50Z
dc.date.available2018-03-28T23:03:50Z
dc.identifierCell Biology International. Academic Press Ltd Elsevier Science Ltd, v. 29, n. 6, n. 458, n. 471, 2005.
dc.identifier1065-6995
dc.identifierWOS:000231189200008
dc.identifier10.1016/j.cellbi.2004.11.025
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/73349
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/73349
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/73349
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1268051
dc.descriptionDuring pregnancy, an interpubic ligament is formed in the mouse pubic symphysis, In late stages, this ligament undergoes "relaxation" to allow proper delivery, which is expected on the 19th day, Proteoglycans and hyaluronic acid play an important role in the remodeling of the extracelfular matrix in these tissues. Gl cosarilinogl cans and protcogl cans were studied by electron microscopic, immunohistochemical and biochemical methods in samples of mouse pubic symphysis from the 12th to 18th day of pregnancy. At the ultrastructural level, using cuprolinic blue and enzymatic digestion by chondroitin lyases, two types of proteoglycan filaments were observed in the fibrocartilage on the 12th day, as well Lis in D15, D17 and D18 pubic ligaments. The only sulfated glycosaminoglycan in these filaments was chondroitin sulfate. as shown by chondroitin lyase treatment. Their electrophoretic mobility, before and after enzymatic degradation. corroborated this inference, The ratio of chondroitin Sulfate dry weight of symphysis showed two phases of increase: between D12 and D15. and between D17 and D18 WC Suggest that the first corresponds mainly to an increase in decorin when the ligament is formed, and the second to versican, during "relaxation". Versican and hyaluronic acid. working as water holding molecules would be responsible for the hydration of the ligament at the end of pregnancy, allowing an increase in resiliency. The presence of hyaluronic acid was confirmed by labeling with HA-probe in the perichondrium, fibrocartilage and ligament. The role of collagen fibers as physical restrictors of the complete expansion of glycosaminoglycans and hyaluronic acid in tissue is discussed. (c) 2005 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.
dc.description29
dc.description6
dc.description458
dc.description471
dc.languageen
dc.publisherAcademic Press Ltd Elsevier Science Ltd
dc.publisherLondon
dc.publisherInglaterra
dc.relationCell Biology International
dc.relationCell Biol. Int.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectproteoglycan
dc.subjecthyaluronic acid
dc.subjectpubic symphysis
dc.subjectpregnancy
dc.subjectCollagen-fibers
dc.subjectRelaxin
dc.subjectGlycosaminoglycans
dc.subjectLigament
dc.subjectDecorin
dc.subjectMice
dc.subjectMetabolism
dc.subjectSteroids
dc.subjectTensile
dc.titleUltrastructural, immunohistochemical and biochemical analysis of glycosarninoglycans and proteoglycans in the mouse, pubic symphysis during pregnancy
dc.typeArtículos de revistas


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