Activity of antioxidant enzymes in response to cadmium in Crotalaria juncea

dc.creatorPereira, GJG
dc.creatorMolina, SMG
dc.creatorLea, PJ
dc.creatorAzevedo, RA
dc.date2002
dc.dateFEB
dc.date2014-11-16T09:06:41Z
dc.date2015-11-26T16:21:35Z
dc.date2014-11-16T09:06:41Z
dc.date2015-11-26T16:21:35Z
dc.date.accessioned2018-03-28T23:03:33Z
dc.date.available2018-03-28T23:03:33Z
dc.identifierPlant And Soil. Springer, v. 239, n. 1, n. 123, n. 132, 2002.
dc.identifier0032-079X
dc.identifierWOS:000175627700011
dc.identifier10.1023/A:1014951524286
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/54175
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/54175
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/54175
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1267976
dc.descriptionThe effects of the heavy metal Cadmium (Cd) on the growth and the activities of the antioxidant enzymes, catalase (CAT. EC 1.11.1.6), Superoxide dismutase (SOD, EC 1.15.1.1) and glutathione reductase (GR. EC 1.6.4.2) have been investigated in Crotalaria juncea seedlings. Concentrations above 0.2 mM CdCl(2) were shown to inhibit strongly the growth of roots and shoots. Cd was shown to accumulate to very high concentrations in the roots, whilst in the leaves. the maximum concentration obtained following treatment with 2 mM CdCl(2). was only 6% of that determined in the roots. Although CAT activity did not exhibit an, major variation in the roots following CdCl(2) treatment, 2 mM CdCl(2) induced a 6-fold increase in activity in the leaves when compared to the untreated control. Non-denaturing PAGE gels stained for SOD activity revealed four isoenzymes, two Mn-SOD and two Cu/ZnSOD. The results observed for SOD were different of those observed for CAT activity. since in both. leaves and roots. no significant changes in the total activity or of the four isoenzymes were observed following the treatment with CdCl(2). GR activity exhibited a similar pattern of that of CAT activity. The concentration of 2 mM CdCl(2) induced a small increase in activity in the roots after 48 h of exposure. whereas in leaves a 7-fold increase in GR activity was detected after 48 hr exposure to 2 mM CdCl(2). The results suggest that in C. juncea the reactive oxygen species (ROS) induced by Cd, are metabolised by CAT in the peroxisomes. In the case of GR activity. the increase observed in the leaves suggest that GR is also playing a role in the detoxification of Cd-induced ROS possibly via the glutathione-ascorbate cycle.
dc.description239
dc.description1
dc.description123
dc.description132
dc.languageen
dc.publisherSpringer
dc.publisherDordrecht
dc.publisherHolanda
dc.relationPlant And Soil
dc.relationPlant Soil
dc.rightsfechado
dc.rightshttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.sourceWeb of Science
dc.subjectcadmium
dc.subjectcatalase
dc.subjectCrotalaria juncea
dc.subjectglutathione reductase
dc.subjectheahy metals
dc.subjectsuperoxide dismutase
dc.subjectCatalase-deficient Mutant
dc.subjectBean Phaseolus-vulgaris
dc.subjectGlutathione-reductase
dc.subjectSuperoxide-dismutase
dc.subjectSoybean Leghemoglobin
dc.subjectNitrogen Assimilation
dc.subjectLipid-peroxidation
dc.subjectTransgenic Plants
dc.subjectBarley Seedlings
dc.subjectBrassica-juncea
dc.titleActivity of antioxidant enzymes in response to cadmium in Crotalaria juncea
dc.typeArtículos de revistas


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