Kinetic studies on veratryl alcohol transformation by horseradish peroxidase

dc.creatorDuran, N
dc.creatorBromberg, N
dc.creatorKunz, A
dc.date2001
dc.dateAPR
dc.date2014-11-16T08:42:59Z
dc.date2015-11-26T16:21:20Z
dc.date2014-11-16T08:42:59Z
dc.date2015-11-26T16:21:20Z
dc.date.accessioned2018-03-28T23:03:21Z
dc.date.available2018-03-28T23:03:21Z
dc.identifierJournal Of Inorganic Biochemistry. Elsevier Science Inc, v. 84, n. 41732, n. 279, n. 286, 2001.
dc.identifier0162-0134
dc.identifierWOS:000168678000014
dc.identifier10.1016/S0162-0134(01)00175-1
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/60984
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/60984
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/60984
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1267934
dc.descriptionA number of peroxidases, such as lignin peroxidase and manganese peroxidase have proved to be useful for industrial applications. Some studies on the effects of temperature and pH stability have been carried out. It is known that veratryl alcohol increases their stability in the range 28-50 degreesC and is oxidized, leading to veratryl aldehyde formation. Similar results with horseradish peroxidase (HRP) in the presence of cofactors were found, but the oxidation of veratryl alcohol in the absence of cofactors was extremely labile at acid pH and inactivated in a few minutes. Considering the growing industrial application of HRP, knowledge of its stability and denaturation kinetics is required. In this study, horseradish peroxidase pool (HRP-VI) and its isoenzymes HRP-VIII (acid) and HRP-IX (basic) have been shown to catalyze the oxidation of veratryl alcohol to veratryl aldehyde in the presence of hydrogen peroxide at pH 5.8 in the 35-45 degreesC range and in the absence of any cofactors. Heat and pH denaturation experiments in the presence and absence of veratryl alcohol incubation were conducted with HRP-VI and HRP-IX isoenzymes. HRP-IX was the most active isoenzyme acting on veratryl alcohol but HRP-VI was the most stable for the temperature range tested. At 35 degreesC the HRP pool presented decay constant (K-d) values of 5.5 x 10(-2) h(-1) and 1.4 10(-2) h(-1) in the absence and presence of veratryl alcohol, respectively. with an effective ratio of 3.9. These results present a new feature of peroxidases that opens one more interesting application of HRP to industrial processes. (C) 2001 Elsevier Science B.V. All rights reserved.
dc.description84
dc.description41732
dc.description279
dc.description286
dc.languageen
dc.publisherElsevier Science Inc
dc.publisherNew York
dc.publisherEUA
dc.relationJournal Of Inorganic Biochemistry
dc.relationJ. Inorg. Biochem.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectperoxidase
dc.subjecthorseradish peroxidase
dc.subjectlignin peroxidase
dc.subjectkinetics
dc.subjectveratryl alcohol
dc.subjectHeat-denaturation Kinetics
dc.subjectPhanerochaete-chrysosporium
dc.subjectLignin Peroxidase
dc.subjectChrysonilia-sitophila
dc.subjectOxidation
dc.subjectStability
dc.subjectPh
dc.subjectPolymerization
dc.subjectPurification
dc.subjectTemperature
dc.titleKinetic studies on veratryl alcohol transformation by horseradish peroxidase
dc.typeArtículos de revistas


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