MMP-2 Regulates Rat Ventral Prostate Development In Vitro

dc.creatorBruni-Cardoso, A
dc.creatorRosa-Ribeiro, R
dc.creatorPascoal, VDB
dc.creatorDe Thomaz, AA
dc.creatorCesar, CL
dc.creatorCarvalho, HF
dc.date2010
dc.dateMAR
dc.date2014-11-16T07:56:36Z
dc.date2015-11-26T16:21:16Z
dc.date2014-11-16T07:56:36Z
dc.date2015-11-26T16:21:16Z
dc.date.accessioned2018-03-28T23:03:20Z
dc.date.available2018-03-28T23:03:20Z
dc.identifierDevelopmental Dynamics. Wiley-liss, v. 239, n. 3, n. 737, n. 746, 2010.
dc.identifier1058-8388
dc.identifierWOS:000275633100002
dc.identifier10.1002/dvdy.22222
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/57163
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/57163
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/57163
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1267927
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionWe have hypothesized that epithelial growth, branching, and canalization in the rodent ventral prostate (VP) would require matrix remodeling, and hence matrix metalloproteinase (MMP) activity. Therefore, the aim of this study was to evaluate the impact of blocking MMP-2, using whole organ culture. siRNA was employed to inhibit MMP-2 expression, and this was compared to GM6001's (a broad-spectrum MMP inhibitor) inhibition of general MMPs. These blocks impaired VP morphogenesis. MMP-2 silencing reduced organ size, epithelial area, and the number of tips, as well as caused a dilation of the distal parts of the epithelium. Histology, 3-D reconstruction, biochemistry, and second harmonic generation (SHG) revealed that MMP-2 silencing affected VP architecture by interfering in epithelial cell proliferation, lumen formation, and cellular organization of both epithelium and stroma, besides intense accumulation of collagen fibers. These data suggest that MMP-2 plays important roles in prostate growth, being directly involved with epithelial morphogenesis. Developmental Dynamics 239:737-746, 2010. (C) 2010 Wiley-Liss, Inc.
dc.description239
dc.description3
dc.description737
dc.description746
dc.descriptionState of Sao Paulo Funding Agency
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFAPESP [07/07564-6]
dc.languageen
dc.publisherWiley-liss
dc.publisherHoboken
dc.publisherEUA
dc.relationDevelopmental Dynamics
dc.relationDev. Dyn.
dc.rightsfechado
dc.rightshttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.sourceWeb of Science
dc.subjectprostate development, MMP, siRNA, GM6001
dc.subjectcollagen
dc.subjectSHG
dc.subjectBranching Morphogenesis
dc.subjectMouse Prostate
dc.subjectMatrix Metalloproteinases
dc.subjectGene-expression
dc.subjectPostnatal-development
dc.subjectAndrogen Regulation
dc.subjectGland Development
dc.subjectCancer
dc.subjectEpithelium
dc.subjectCells
dc.titleMMP-2 Regulates Rat Ventral Prostate Development In Vitro
dc.typeArtículos de revistas


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