Lonomia obliqua caterpillar venom increases permeability of the blood-brain barrier in rats

dc.creatorda Silva, GH
dc.creatorHyslop, S
dc.creatorda Cruz-Hofling, MA
dc.date2004
dc.dateNOV
dc.date2014-11-16T08:26:17Z
dc.date2015-11-26T16:21:13Z
dc.date2014-11-16T08:26:17Z
dc.date2015-11-26T16:21:13Z
dc.date.accessioned2018-03-28T23:03:17Z
dc.date.available2018-03-28T23:03:17Z
dc.identifierToxicon. Pergamon-elsevier Science Ltd, v. 44, n. 6, n. 625, n. 634, 2004.
dc.identifier0041-0101
dc.identifierWOS:000225003200006
dc.identifier10.1016/j.toxicon.2004.07.016
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/52662
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/52662
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/52662
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1267916
dc.descriptionHuman envenoming by caterpillars of the saturniid moth Lonomia obliqua in southern Brazil produces a mild local response (erythema, some edema, and pain) and systemic effects that include incoagulable blood, renal failure and in severe accidents intracerebral hemorrhage. In this work, we used light and electron microscopy to investigate the morphological alterations in the brain and blood-brain barrier of rats injected intravenously with venom from L. obliqua spicules (200 mug/kg). Five semi-purified fractions of venom (200 mug/kg each) were also assayed. Quantitative morphological and ultrastructural analyses were done 6, 18, 24 and 72 h after the i.v. injection of venom and its fractions. Light microscopy showed that 6 h after envenoming there was cerebellar edema, which decreased by 72 h. Intracerebral hemorrhage occurred in only one rat 24 h after the injection of venom. Blood-brain barrier (BBB) breakdown, assessed by transmission electron microscopy based on the passage of an extracellular tracer (lanthanum nitrate) between brain capillary endothelial cells, was observed in the cerebellum and hippocampus 18 h after venom injection. At this time, the cerebellum was more sensitive to the venom than the hippocampus, as shown by the greater number of leaky vessels. The number of capillaries showing breakdown was lower after 72 h than after 18 h. None of the semi-purified fractions significantly increased the number of leaky vessels. These results indicate that L. obliqua caterpillar venom has a deleterious action on the rat BBB. The lack of effect of the venom fractions when administered alone suggested that a synergistic action of venom components may be responsible for the damage seen in the central nervous system, but this was not confirmed when three combinations of the fractions were tested. (C) 2004 Elsevier Ltd. All rights reserved.
dc.description44
dc.description6
dc.description625
dc.description634
dc.languageen
dc.publisherPergamon-elsevier Science Ltd
dc.publisherOxford
dc.publisherInglaterra
dc.relationToxicon
dc.relationToxicon
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectblood-brain barrier
dc.subjectcapillaries
dc.subjectLonomia obliqua
dc.subjectmorphometry
dc.subjectpermeability
dc.subjecttransmission electron microscopy
dc.subjectvenom
dc.subjectActivator Serine-protease
dc.subjectHorseradish-peroxidase
dc.subjectHemorrhagic Syndrome
dc.subjectBreakdown
dc.subjectProthrombin
dc.subjectContact
dc.subjectEndothelium
dc.subjectTransport
dc.subjectSeizures
dc.subjectLopap
dc.titleLonomia obliqua caterpillar venom increases permeability of the blood-brain barrier in rats
dc.typeArtículos de revistas


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