CALCIUM HOMEOSTASIS IN TRYPANOSOMA-CRUZI AMASTIGOTES - PRESENCE OF INOSITOL PHOSPHATES AND LACK OF AN INOSITOL 1,4,5-TRISPHOSPHATE-SENSITIVE CALCIUM POOL

dc.creatorMORENO, SNJ
dc.creatorVERCESI, AE
dc.creatorPIGNATARO, OP
dc.creatorDOCAMPO, R
dc.date1992
dc.dateJUN
dc.date2014-12-16T11:36:14Z
dc.date2015-11-26T16:20:58Z
dc.date2014-12-16T11:36:14Z
dc.date2015-11-26T16:20:58Z
dc.date.accessioned2018-03-28T23:03:11Z
dc.date.available2018-03-28T23:03:11Z
dc.identifierMolecular And Biochemical Parasitology. Elsevier Science Bv, v. 52, n. 2, n. 251, n. 262, 1992.
dc.identifier0166-6851
dc.identifierWOS:A1992HX35800011
dc.identifier10.1016/0166-6851(92)90057-Q
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/78329
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/78329
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/78329
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1267891
dc.descriptionThe permeabilization of Trypanosoma cruzi amastigotes with digitonin allowed the study of Ca2+ fluxes between intracellular organelles in situ. In addition, fura-2 was used to determine the cytosolic Ca2+ concentration in the intact cells. When amastigotes were permeabilized in a reaction medium containing MgATP, succinate and 3.5-mu-M Ca2+, they lowered the medium Ca2+ concentration to the submicromolar level, a range which correlates favorably with that detected in the intact cells with fura-2. The presence of 1-mu-M FCCP strongly decreased the initial rate of Ca2+ sequestration by these permeabilized cells. This FCCP-insensitive Ca2+ uptake, probably represented by the endoplasmic reticulum, was completely inhibited by 500-mu-M vanadate. On the other hand, when vanadate instead of FCCP was present, the initial rate of Ca2+ accumulation was decreased and the Ca2+ set point was increased to about 0.8-mu-M. The succinate dependence and FCCP sensitivity of the later Ca2+ uptake indicate that it may be exerted by the mitochondria. Despite the presence of inositol phosphates, as determined by [H-3]inositol incorporation, and of a large extramitochondrial Ca2+ pool, no IP3-sensitive or thapsigargin-sensitive Ca2+ release could be detected in either amastigotes or epimastigotes.
dc.description52
dc.description2
dc.description251
dc.description262
dc.languageen
dc.publisherElsevier Science Bv
dc.publisherAmsterdam
dc.publisherHolanda
dc.relationMolecular And Biochemical Parasitology
dc.relationMol. Biochem. Parasitol.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectAMASTIGOTE
dc.subjectCALCIUM
dc.subjectINOSITOL PHOSPHATE
dc.subjectTHAPSIGARGIN
dc.subjectTRYPANOSOMA-CRUZI
dc.subjectFree-radical Metabolites
dc.subjectIndicators Arsenazo-iii
dc.subjectEpidermal Growth-factor
dc.subjectLeydig Tumor-cells
dc.subjectIntracellular Calcium
dc.subjectCa-2+ Transport
dc.subjectMitochondria Insitu
dc.subjectSuperoxide Anion
dc.subjectAntipyrylazo-iii
dc.subjectGeneration
dc.titleCALCIUM HOMEOSTASIS IN TRYPANOSOMA-CRUZI AMASTIGOTES - PRESENCE OF INOSITOL PHOSPHATES AND LACK OF AN INOSITOL 1,4,5-TRISPHOSPHATE-SENSITIVE CALCIUM POOL
dc.typeArtículos de revistas


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