dc.creator | MORENO, SNJ | |
dc.creator | VERCESI, AE | |
dc.creator | PIGNATARO, OP | |
dc.creator | DOCAMPO, R | |
dc.date | 1992 | |
dc.date | JUN | |
dc.date | 2014-12-16T11:36:14Z | |
dc.date | 2015-11-26T16:20:58Z | |
dc.date | 2014-12-16T11:36:14Z | |
dc.date | 2015-11-26T16:20:58Z | |
dc.date.accessioned | 2018-03-28T23:03:11Z | |
dc.date.available | 2018-03-28T23:03:11Z | |
dc.identifier | Molecular And Biochemical Parasitology. Elsevier Science Bv, v. 52, n. 2, n. 251, n. 262, 1992. | |
dc.identifier | 0166-6851 | |
dc.identifier | WOS:A1992HX35800011 | |
dc.identifier | 10.1016/0166-6851(92)90057-Q | |
dc.identifier | http://www.repositorio.unicamp.br/jspui/handle/REPOSIP/78329 | |
dc.identifier | http://www.repositorio.unicamp.br/handle/REPOSIP/78329 | |
dc.identifier | http://repositorio.unicamp.br/jspui/handle/REPOSIP/78329 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1267891 | |
dc.description | The permeabilization of Trypanosoma cruzi amastigotes with digitonin allowed the study of Ca2+ fluxes between intracellular organelles in situ. In addition, fura-2 was used to determine the cytosolic Ca2+ concentration in the intact cells. When amastigotes were permeabilized in a reaction medium containing MgATP, succinate and 3.5-mu-M Ca2+, they lowered the medium Ca2+ concentration to the submicromolar level, a range which correlates favorably with that detected in the intact cells with fura-2. The presence of 1-mu-M FCCP strongly decreased the initial rate of Ca2+ sequestration by these permeabilized cells. This FCCP-insensitive Ca2+ uptake, probably represented by the endoplasmic reticulum, was completely inhibited by 500-mu-M vanadate. On the other hand, when vanadate instead of FCCP was present, the initial rate of Ca2+ accumulation was decreased and the Ca2+ set point was increased to about 0.8-mu-M. The succinate dependence and FCCP sensitivity of the later Ca2+ uptake indicate that it may be exerted by the mitochondria. Despite the presence of inositol phosphates, as determined by [H-3]inositol incorporation, and of a large extramitochondrial Ca2+ pool, no IP3-sensitive or thapsigargin-sensitive Ca2+ release could be detected in either amastigotes or epimastigotes. | |
dc.description | 52 | |
dc.description | 2 | |
dc.description | 251 | |
dc.description | 262 | |
dc.language | en | |
dc.publisher | Elsevier Science Bv | |
dc.publisher | Amsterdam | |
dc.publisher | Holanda | |
dc.relation | Molecular And Biochemical Parasitology | |
dc.relation | Mol. Biochem. Parasitol. | |
dc.rights | fechado | |
dc.rights | http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy | |
dc.source | Web of Science | |
dc.subject | AMASTIGOTE | |
dc.subject | CALCIUM | |
dc.subject | INOSITOL PHOSPHATE | |
dc.subject | THAPSIGARGIN | |
dc.subject | TRYPANOSOMA-CRUZI | |
dc.subject | Free-radical Metabolites | |
dc.subject | Indicators Arsenazo-iii | |
dc.subject | Epidermal Growth-factor | |
dc.subject | Leydig Tumor-cells | |
dc.subject | Intracellular Calcium | |
dc.subject | Ca-2+ Transport | |
dc.subject | Mitochondria Insitu | |
dc.subject | Superoxide Anion | |
dc.subject | Antipyrylazo-iii | |
dc.subject | Generation | |
dc.title | CALCIUM HOMEOSTASIS IN TRYPANOSOMA-CRUZI AMASTIGOTES - PRESENCE OF INOSITOL PHOSPHATES AND LACK OF AN INOSITOL 1,4,5-TRISPHOSPHATE-SENSITIVE CALCIUM POOL | |
dc.type | Artículos de revistas | |