Protease production during growth and autolysis of submerged Metarhizum anisopliae cultures

dc.creatorBraga, GUL
dc.creatorDestefano, RHR
dc.creatorMessias, CL
dc.date1999
dc.dateAPR-JUN
dc.date2014-12-02T16:25:48Z
dc.date2015-11-26T16:20:19Z
dc.date2014-12-02T16:25:48Z
dc.date2015-11-26T16:20:19Z
dc.date.accessioned2018-03-28T23:02:48Z
dc.date.available2018-03-28T23:02:48Z
dc.identifierRevista De Microbiologia. Soc Brasileira Microbiologia, v. 30, n. 2, n. 107, n. 113, 1999.
dc.identifier0001-3714
dc.identifierWOS:000083410000004
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/58293
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/58293
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/58293
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1267794
dc.descriptionThe growth and autolysis of two strains of the entomopathogenic deuteromycete fungus Metarhizium anisopliae var. anisopliae were evaluated in medium containing casein or glucose as carbon source. Parameters such as economic coefficient and degree of autolysis were determined for each strain. Protease production was determined throughout the growth and autolysis phases of the cultures on medium under conditions of protease induction (in the presence of casein as sole source of carbon and nitrogen). The fungus was shown to utilize casein as a carbon/energy source in a more efficient manner than glucose. The autolysis shown by the strains was intense under both types of growth conditions, reaching up to 62.7% of the dry mass produced and started soon after the depletion of the exogenous carbon source. The relationship between the proteolytic activities of the two strains evaluated varied significantly (a maximum of 19.78 on the 5(th) day and a minimum of 2.03 on the 16(th) day of growth) during the various growth and autolysis phases, clearly showing that the difference between the growth curves and the difference in the kinetics of enzyme production may decisively affect the process of strain selection for protease production.
dc.description30
dc.description2
dc.description107
dc.description113
dc.languageen
dc.publisherSoc Brasileira Microbiologia
dc.publisherSao Paulo
dc.publisherBrasil
dc.relationRevista De Microbiologia
dc.relationRev. Microbiol.
dc.rightsaberto
dc.sourceWeb of Science
dc.subjectMetarhizium anisopliae
dc.subjectprotease production
dc.subjectfungal growth
dc.subjectfungal autolysis
dc.subjectentomopathogenic fungus
dc.subjectGalleria-mellonella Larvae
dc.subjectExtracellular Enzymes
dc.subjectManduca-sexta
dc.subjectCuticle
dc.subjectPenetration
dc.titleProtease production during growth and autolysis of submerged Metarhizum anisopliae cultures
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución