Mitochondrial permeability transition induced by chemically generated singlet oxygen

dc.creatorCosso, RG
dc.creatorTurim, J
dc.creatorNantes, IL
dc.creatorAlmeida, AM
dc.creatorMascio, PD
dc.creatorVercesi, AE
dc.date2002
dc.dateJUN
dc.date2014-11-16T05:44:54Z
dc.date2015-11-26T16:20:13Z
dc.date2014-11-16T05:44:54Z
dc.date2015-11-26T16:20:13Z
dc.date.accessioned2018-03-28T23:02:45Z
dc.date.available2018-03-28T23:02:45Z
dc.identifierJournal Of Bioenergetics And Biomembranes. Kluwer Academic/plenum Publ, v. 34, n. 3, n. 157, n. 163, 2002.
dc.identifier0145-479X
dc.identifierWOS:000176461800002
dc.identifier10.1023/A:1016075218162
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/57125
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/57125
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/57125
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1267779
dc.descriptionPure singlet molecular oxygen (O-1(2)) generated by thermal decomposition of the 3,3'-(1,4-naphthylidene) dipropionate endoperoxide (NDPO2), inhibited respiration of isolated rat liver mitochondria supported by NADH-linked substrates or succinate, but not by N,N,N,N-tetramehyl-p-phenylene-diamine (TMPD)/ascorbate. Under the latter conditions, mitochondria treated with 2.7 mM NDPO2 exhibited a decrease in transmembrane potential (DeltaPsi) in manner dependent on NDPO2 exposure time. This process was sensitive to the mitochondrial permeability transition inhibitors EGTA, dithiothreitol, ADP, and cyclosporin A. The presence of deuterium oxide (D2O), that increases O-1(2) lifetime, significantly enhanced NDPO2-promoted mitochondrial permeabilization. In addition, NDPO2-induced mitochondrial permeabilization was accompanied by DTT or ADP-sensitive membrane protein thiol oxidation. Taken together, these results provide evidence that mitochondrial permeability transition induced by chemically generated singlet oxygen is mediated by the oxidation of membrane protein thiols.
dc.description34
dc.description3
dc.description157
dc.description163
dc.languageen
dc.publisherKluwer Academic/plenum Publ
dc.publisherNew York
dc.publisherEUA
dc.relationJournal Of Bioenergetics And Biomembranes
dc.relationJ. Bioenerg. Biomembr.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectrat liver mitochondria
dc.subjectsingle oxygen
dc.subjectpermeability transition
dc.subjectNDPO2
dc.subjectThiol Cross-linking
dc.subjectPhotodynamic-action
dc.subjectPlus Prooxidants
dc.subjectMembrane Permeabilization
dc.subjectNaphthalene Endoperoxides
dc.subjectLipid-peroxidation
dc.subjectOxidative Stress
dc.subjectPhosphorylation
dc.subjectHemoproteins
dc.subjectMechanism
dc.titleMitochondrial permeability transition induced by chemically generated singlet oxygen
dc.typeArtículos de revistas


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