Transcriptional analysis of the human PAX9 promoter

dc.creatorde Almeida, CV
dc.creatorde Andrade, SC
dc.creatorSaito, CPB
dc.creatorRamenzoni, LL
dc.creatorLine, SRP
dc.date2010
dc.dateSEP-OCT
dc.date2014-11-15T22:51:28Z
dc.date2015-11-26T16:16:46Z
dc.date2014-11-15T22:51:28Z
dc.date2015-11-26T16:16:46Z
dc.date.accessioned2018-03-28T23:01:43Z
dc.date.available2018-03-28T23:01:43Z
dc.identifierJournal Of Applied Oral Science. Univ Sao Paulo Fac Odontologia Bauru, v. 18, n. 5, n. 482, n. 486, 2010.
dc.identifier1678-7757
dc.identifierWOS:000284590700009
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/77520
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/77520
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/77520
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1267528
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionObjectives: PAX9 belongs to the Pax family of transcriptional factor genes. This gene is expressed in embryonic tissues such as somites, pharyngeal pouch endoderm, distal limb buds and neural crest-derived mesenchyme. Polymorphisms in the upstream promoter region of the human PAX9 have been associated with human non-syndromic tooth agenesis. In the present study, we verified the in vitro mRNA expression of this gene and the luciferase activity of two constructs containing promoter sequences of the PAX9 gene. Material and Methods: Embryonic tissues were obtained from digits, face, and midbrain/hindbrain regions. Fragments containing PAX9 promoter sequences were cloned into reporter plasmids and were transfected into the different cell cultures. mRNA were extracted from primary cell cultures. Results: The semi-quantitative RT-PCR results showed that in vitro E13.5 limb bud and CNS cells express PAX9, but cells derived from the facial region do not. Moreover, the luciferase assay showed that protein activity of the constructed vector was weaker than pgl3-basic alone. Conclusions: The present results suggest that the promoter sequences analyzed are not sufficient to drive PAX9 gene transcription.
dc.description18
dc.description5
dc.description482
dc.description486
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageen
dc.publisherUniv Sao Paulo Fac Odontologia Bauru
dc.publisherBauru-sp
dc.publisherBrasil
dc.relationJournal Of Applied Oral Science
dc.relationJ. Appl. Oral Sci.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectPAX9 transcription factor
dc.subjectGenetic promoter regions
dc.subjectAnodontia
dc.subjectAutosomal-dominant Hypodontia
dc.subjectMolar Oligodontia
dc.subjectNonsense Mutation
dc.subjectMissense Mutation
dc.subjectTooth Development
dc.subjectGene
dc.subjectIdentification
dc.subjectExpression
dc.subjectAgenesis
dc.titleTranscriptional analysis of the human PAX9 promoter
dc.typeArtículos de revistas


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