| dc.creator | Macedo, MLR | |
| dc.creator | de Matos, DGG | |
| dc.creator | Machado, OLT | |
| dc.creator | Marangoni, S | |
| dc.creator | Novello, JC | |
| dc.date | 2000 | |
| dc.date | JUL | |
| dc.date | 2014-12-02T16:25:26Z | |
| dc.date | 2015-11-26T16:16:44Z | |
| dc.date | 2014-12-02T16:25:26Z | |
| dc.date | 2015-11-26T16:16:44Z | |
| dc.date.accessioned | 2018-03-28T23:01:43Z | |
| dc.date.available | 2018-03-28T23:01:43Z | |
| dc.identifier | Phytochemistry. Pergamon-elsevier Science Ltd, v. 54, n. 6, n. 553, n. 558, 2000. | |
| dc.identifier | 0031-9422 | |
| dc.identifier | WOS:000088715000001 | |
| dc.identifier | 10.1016/S0031-9422(00)00155-2 | |
| dc.identifier | http://www.repositorio.unicamp.br/jspui/handle/REPOSIP/77043 | |
| dc.identifier | http://www.repositorio.unicamp.br/handle/REPOSIP/77043 | |
| dc.identifier | http://repositorio.unicamp.br/jspui/handle/REPOSIP/77043 | |
| dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1267525 | |
| dc.description | A trypsin inhibitor from Dimorphandra mollis seeds was isolated to apparent homogeneity by a combination of ammonium sulfate precipitation, gel filtration, ion-exchange and affinity chromatographic techniques. SDS-PAGE analysis gave an apparent molecular weight of 20 kDa, and isoelectric focusing analysis demonstrated the presence of three isoforms. The partial N-terminal amino acid sequence of the purified protein showed a high degree of homology with various members of the Kunitz family of inhibitors. This inhibitor, which inhibited trypsin activity with a K-i of 5.3 x 10(-10) M, is formed by a single polypeptide chain with an arginine residue in the reactive site. (C) 2000 Elsevier Science Ltd. All rights reserved. | |
| dc.description | 54 | |
| dc.description | 6 | |
| dc.description | 553 | |
| dc.description | 558 | |
| dc.language | en | |
| dc.publisher | Pergamon-elsevier Science Ltd | |
| dc.publisher | Oxford | |
| dc.publisher | Inglaterra | |
| dc.relation | Phytochemistry | |
| dc.relation | Phytochemistry | |
| dc.rights | fechado | |
| dc.rights | http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy | |
| dc.source | Web of Science | |
| dc.subject | Dimorphandra mollis | |
| dc.subject | leguminosae | |
| dc.subject | trypsin inhibitor | |
| dc.subject | Kunitz inhibitor | |
| dc.subject | N-terminal sequence | |
| dc.subject | Proteinase-inhibitors | |
| dc.subject | Leguminosae | |
| dc.title | Trypsin inhibitor from Dimorphandra mollis seeds: purification and properties | |
| dc.type | Artículos de revistas | |
