Optimizing the production of cutinase by Fusarium oxysporum using response surface methodology

dc.creatorPio, TF
dc.creatorMacedo, GA
dc.date2007
dc.dateOCT 1
dc.date2014-11-15T19:27:00Z
dc.date2015-11-26T16:13:35Z
dc.date2014-11-15T19:27:00Z
dc.date2015-11-26T16:13:35Z
dc.date.accessioned2018-03-28T23:01:05Z
dc.date.available2018-03-28T23:01:05Z
dc.identifierEnzyme And Microbial Technology. Elsevier Science Inc, v. 41, n. 5, n. 613, n. 619, 2007.
dc.identifier0141-0229
dc.identifierWOS:000249224400010
dc.identifier10.1016/j.enzmictec.2007.05.008
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/69559
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/69559
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/69559
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1267370
dc.descriptionIn the present study, the authors measured the production of cutinase by Fusarium oxysporum in the presence of several carbon and nitrogen sources (glycides, fatty acids and oils, and several organic and inorganic nitrogen sources), trying to find a cost-effective substitute for cutin in the culture medium as an inducer of cutinase production. The results were evaluated by the Tukey test, and flaxseed oil was found to give the best results as a cutinase inducer. Optimization of the growth medium was carried out using response surface methodology, aiming at optimizing the concentrations of the key components. The experimental results were fitted to a second-order polynomial model at a 95% level of significance (P < 0.05). The greatest cutinolytic activity was obtained in a liquid mineral medium supplemented with flaxseed oil, showing an increase in enzymatic activity from 11 to 22.68 mu Mol/min mL after 48 h of fermentation. The results support the use of flaxseed oil as a substitute for cutin, which is difficult and expensive to obtain, for the production of cutinase in a larger scale. (C) 2007 Elsevier Inc. All rights reserved.
dc.description41
dc.description5
dc.description613
dc.description619
dc.languageen
dc.publisherElsevier Science Inc
dc.publisherNew York
dc.publisherEUA
dc.relationEnzyme And Microbial Technology
dc.relationEnzyme Microb. Technol.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectcutinase
dc.subjectfusarium osysporum
dc.subjectmedia optimization
dc.subjectfractional factorial design
dc.subjectcentral composite design
dc.subjectTransesterification Reactions
dc.subjectOrganic-solvents
dc.subjectOptimization
dc.subjectStability
dc.subjectMicelles
dc.subjectLipase
dc.subjectDesign
dc.titleOptimizing the production of cutinase by Fusarium oxysporum using response surface methodology
dc.typeArtículos de revistas


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