Cholesteryl ester transfer protein (CETP) increases postprandial triglyceridaemia and delays triacylglycerol plasma clearance in transgenic mice

dc.creatorSalerno, AG
dc.creatorPatricio, PR
dc.creatorBerti, JA
dc.creatorOliveira, HCF
dc.date2009
dc.dateMAY 1
dc.date2014-11-15T18:38:49Z
dc.date2015-11-26T16:13:13Z
dc.date2014-11-15T18:38:49Z
dc.date2015-11-26T16:13:13Z
dc.date.accessioned2018-03-28T23:01:02Z
dc.date.available2018-03-28T23:01:02Z
dc.identifierBiochemical Journal. Portland Press Ltd, v. 419, n. 629, n. 634, 2009.
dc.identifier0264-6021
dc.identifierWOS:000265901500012
dc.identifier10.1042/BJ20081299
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/55835
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/55835
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/55835
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1267357
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionThe CETP (cholesteryl ester transfer protein) is a plasma protein synthesized in several tissues, mainly in the liver; CETP reduces plasma HDL (high-density lipoprotein) cholesterol and increases the risk of atherosclerosis. The effect of CETP levels on postprandial intravascular metabolism of TAGs (triacylglycerols) is an often-overlooked aspect of the relationship between CETP and lipoprotein metabolism. Here, we tested the hypothesis that CETP delays the plasma clearance of TAG-rich lipoprotein by comparing human CETP expressing Tg (transgenic) and non-Tg mice. After an oral fat load, the postprandial triglyceridaemia curve was markedly increased in CETP-Tg compared with non-Tg mice (280 +/- 30 versus 190 +/- 20 mg/dl per 6 h respectively, P < 0.02). No differences in intestinal fat absorption and VLDL (very-low-density lipoprotein) secretion rates were observed. Kinetic studies of double-labelled chylomicron-like EMs (emulsions) showed that both [(3)H]triolein and [(14)C] cholesteryl oleate FCRs (fractional clearance rates) were significantly reduced (similar to 20%) in CETP-Tg mice. Furthermore, TAG from lipid EM pre-incubated with CETP-Tg plasma had plasma clearance and liver uptake significantly lower than the non-Tg plasma-treated lipid EM. In addition, reductions in post-heparin plasma LPL (lipoprotein lipase) activity (50%) and adipose tissue mRNA abundance (39%) were verified in CETP-Tg mice. Therefore we conclude that CETP expression in Tg mice delays plasma clearance and liver uptake of TAG-rich lipoproteins by two mechanisms: (i) transferring TAG to HDLs and increasing CE content of the remnant particles and (ii) by diminishing LPL expression. These findings show that the level of CETP expression can influence the responsiveness to dietary fat and may lead to fat intolerance.
dc.description419
dc.description629
dc.description634
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFAPESP [2006/59786-0]
dc.descriptionCNPq [474055/2006-0]
dc.languageen
dc.publisherPortland Press Ltd
dc.publisherLondon
dc.publisherInglaterra
dc.relationBiochemical Journal
dc.relationBiochem. J.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectcholesteryl ester transfer protein (CETP)
dc.subjectfat tolerance
dc.subjectlipoprotein lipase
dc.subjectpostprandial lipoprotein
dc.subjectremnant
dc.subjecttransgenic mice
dc.subjectHigh-density-lipoprotein
dc.subjectCoronary-artery-disease
dc.subjectEmulsion Models
dc.subjectAdipose-tissue
dc.subjectChylomicrons
dc.subjectMetabolism
dc.subjectRemoval
dc.subjectLipemia
dc.subjectAtherosclerosis
dc.subjectDeficiency
dc.titleCholesteryl ester transfer protein (CETP) increases postprandial triglyceridaemia and delays triacylglycerol plasma clearance in transgenic mice
dc.typeArtículos de revistas


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