Chromatin supraorganization and extensibility in mouse hepatocytes following starvation and refeeding

dc.creatorMoraes, AS
dc.creatorVidal, BD
dc.creatorGuaraldo, AMA
dc.creatorMello, MLS
dc.date2005
dc.dateFEB
dc.date2014-11-15T18:56:45Z
dc.date2015-11-26T16:13:11Z
dc.date2014-11-15T18:56:45Z
dc.date2015-11-26T16:13:11Z
dc.date.accessioned2018-03-28T23:01:00Z
dc.date.available2018-03-28T23:01:00Z
dc.identifierCytometry Part A. Wiley-liss, v. 63A, n. 2, n. 94, n. 107, 2005.
dc.identifier1552-4922
dc.identifierWOS:000226858100005
dc.identifier10.1002/cyto.a.20113
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/55842
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/55842
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/55842
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1267351
dc.descriptionBackground: The effect of 48 h of starvation and of 48 h of refeeding subsequent to starvation on chromatin supraorganization and extensibility was studied in hepatocytes from adult mice. Methods: Methods used involved topochemical assays, image analysis, gravity action, and polarization microscopy. Results: Starvation increased the chromatin packing states, especially in areas of noncondensed chromatin, and induced drastic decreases in concanavalin A reactivity due to nuclear matrix glycoproteins and the frequency of nuclei with chromatin extensibility under gravity. Changes in chromatin packing state were accompanied by shifts of nuclear areas of part of the nuclear population to smaller values but did not affect the respective Feulgen-DNA amounts except for a few nuclei. The extent of chromatin unpackaging, but not of frequency of nuclei with formation of extended chromatin fibers, in starved mice that were refed was greater than in well-fed controls. Refeeding induced increase in Feulgen-DNA amounts and regain and redistribution of concanavalin A-reactive nuclear glycoproteins. However, the duration of refeeding used was probably insufficient to reestablish the stereo arrangement of the chromatin-nuclear matrix and to restore chromatin fluidity to the level seen in well-fed mice. Conclusions: The changes in the liver cell nuclei associated with starvation and refeeding of adult mice involved chromatin supraorganization, hepatocyte proliferation (refeeding), and the loss, regain, and redistribution of nuclear proteins, especially nuclear matrix components, related to chromatin organization and extensibility. These changes are suggested as favoring the silencing and reactivation of transcriptional activities, depending on the organism's nutritional state. (C) 2005 Wiley-Liss, Inc.
dc.description63A
dc.description2
dc.description94
dc.description107
dc.languageen
dc.publisherWiley-liss
dc.publisherHoboken
dc.publisherEUA
dc.relationCytometry Part A
dc.relationCytom. Part A
dc.rightsfechado
dc.rightshttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.sourceWeb of Science
dc.subjectchromatin supraorganization
dc.subjectextended chromatin fibers
dc.subjectstarvation
dc.subjectrefeeding
dc.subjectnuclear matrix
dc.subjectIn-situ Hybridization
dc.subjectNuclear-matrix
dc.subjectExtended Chromatin
dc.subjectConcanavalin-a
dc.subjectGene-expression
dc.subjectHistone Content
dc.subjectRat-liver
dc.subjectProteins
dc.subjectCells
dc.subjectDna
dc.titleChromatin supraorganization and extensibility in mouse hepatocytes following starvation and refeeding
dc.typeArtículos de revistas


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