Circulating leukocyte heat shock protein 70 (HSP70) and oxidative stress markers in rats after a bout of exhaustive exercise

dc.creatorAntunes-Neto, JMF
dc.creatorToyama, MH
dc.creatorCarneiro, EM
dc.creatorBoschero, AC
dc.creatorPereira-Da-Silva, L
dc.creatorMacedo, DV
dc.date2006
dc.dateJUN
dc.date2014-11-15T16:37:59Z
dc.date2015-11-26T16:12:25Z
dc.date2014-11-15T16:37:59Z
dc.date2015-11-26T16:12:25Z
dc.date.accessioned2018-03-28T23:00:39Z
dc.date.available2018-03-28T23:00:39Z
dc.identifierStress-the International Journal On The Biology Of Stress. Taylor & Francis Ltd, v. 9, n. 2, n. 107, n. 115, 2006.
dc.identifier1025-3890
dc.identifierWOS:000241211600005
dc.identifier10.1080/10253890600772211
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/55938
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/55938
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/55938
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1267263
dc.descriptionA novel method to measure oxidative stress resulting from exhaustive exercise in rats is presented. In this new procedure we evaluated the erythrocyte antioxidant enzymes, catalase ( CAT) and glutathione reductase (GR), the plasma oxidative attack markers, reactive carbonyl derivatives (RCD) and thiobarbituric reactive substances (TBARS). Muscular tissue damage was evaluated by monitoring plasma creatine kinase (CK) and plasma taurine ( Tau) concentrations. Also, we monitored total sulphydryl groups (TSG) and uric acid (UA), and the level of the 70 kDa heat shock protein (HSP70) in leukocytes as a marker of oxidative stress. In the study we found a correspondence between erythrocyte CAT and GR activities and leukocyte HSP70 levels, principally 3 h after the acute exercise, and this suggested an integrated mechanism of antioxidant defense. The increase in levels of plasma Tau was coincident with the increasing plasma levels of CK and TBARS, principally after two hours of exercise. Thus tissue damage occurred before the expression of any anti-oxidant system markers and the monitoring of Tau, CK or TBARS may be important for the estimation of oxidative stress during exhaustive exercise. Furthermore, the integrated analyses could be of value in a clinical setting to quantify the extent of oxidative stress risk and reduce the need to perform muscle biopsies as a tool of clinical evaluation.
dc.description9
dc.description2
dc.description107
dc.description115
dc.languageen
dc.publisherTaylor & Francis Ltd
dc.publisherAbingdon
dc.publisherInglaterra
dc.relationStress-the International Journal On The Biology Of Stress
dc.relationStress
dc.rightsfechado
dc.rightshttp://journalauthors.tandf.co.uk/permissions/reusingOwnWork.asp
dc.sourceWeb of Science
dc.subjectexercise
dc.subjectfree amino acids
dc.subjectheat shock protein
dc.subjectoxidative stress
dc.subjectSkeletal-muscle
dc.subjectEccentric Exercise
dc.subjectLipid-peroxidation
dc.subjectAmino-acids
dc.subjectAntioxidant Capacity
dc.subjectEnzyme-activities
dc.subjectOxygen Radicals
dc.subjectTissue-damage
dc.subjectSoleus Muscle
dc.subjectDelayed-onset
dc.titleCirculating leukocyte heat shock protein 70 (HSP70) and oxidative stress markers in rats after a bout of exhaustive exercise
dc.typeArtículos de revistas


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