Effects of polyoxyethylene chain length on erythrocyte hemolysis induced by poly[oxyethylene(n)nonylphenol] non-ionic surfactants

dc.creatorGalembeck, E
dc.creatorAlonso, A
dc.creatorMeirelles, NC
dc.date1998
dc.dateMAY 15
dc.date2014-12-02T16:24:56Z
dc.date2015-11-26T16:11:30Z
dc.date2014-12-02T16:24:56Z
dc.date2015-11-26T16:11:30Z
dc.date.accessioned2018-03-28T22:59:59Z
dc.date.available2018-03-28T22:59:59Z
dc.identifierChemico-biological Interactions. Elsevier Sci Ireland Ltd, v. 113, n. 2, n. 91, n. 103, 1998.
dc.identifier0009-2797
dc.identifierWOS:000075051500001
dc.identifier10.1016/S0009-2797(98)00006-4
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/65059
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/65059
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/65059
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1267104
dc.descriptionThe effects of three different poly[oxyethylene (n) nonylphenols], n = 9.5, 20 and 100 oxyethylene (EO) units, on erythrocyte hemolysis and on the fluidity of the erythrocyte membrane were studied. The three different surfactants showed different effects. The surfactant with average n = 9.5 EO units (C9E9) shows a biphasic effect: at low concentrations it protects erythrocytes against hypotonic hemolysis, but at higher concentrations it induces hemolysis both in isotonic and hypotonic buffers. C9E20 does not affect the erythrocyte membrane resistance to hemolysis, independent of the buffer osmolarity; this detergent did not show a hemolytic effect. C9E100 is an effective protective agent against hypotonic hemolysis, in concentrations > 2 x 10(-4) M. EPR spectroscopy of spin-labeled stearic acid indicated that the three different surfactants increase the fluidity of erythrocyte ghost membranes. At the higher C9E20 and C9E100 surfactant concentrations in the presence of membrane ghosts, spin-label is located in the surfactant micelles. In the case of the hemolytic concentrations of C9E9, mixed (surfactant plus phospholipid) micelles are formed. These results suggest that C9E9 has a higher affinity for membrane phospholipids, which accounts for its lytic activity. The protective effect of C9E100 is assigned to the osmotic buffering of the liquid surrounding the cell membrane, due to the large polar chains anchored to the membrane outer monolayer but other mechanisms previously considered in the literature may also be effective. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.
dc.description113
dc.description2
dc.description91
dc.description103
dc.languageen
dc.publisherElsevier Sci Ireland Ltd
dc.publisherClare
dc.publisherIrlanda
dc.relationChemico-biological Interactions
dc.relationChem.-Biol. Interact.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectnon-ionic surfactant
dc.subjecthemolysis
dc.subjectEPR
dc.subjectRenex surfactants
dc.subjectmembrane-surfactant bidding
dc.subjectMembranes
dc.subjectReconstitution
dc.subjectLiposomes
dc.titleEffects of polyoxyethylene chain length on erythrocyte hemolysis induced by poly[oxyethylene(n)nonylphenol] non-ionic surfactants
dc.typeArtículos de revistas


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