Cold-induced PGC-1 alpha expression modulates muscle glucose uptake through an insulin receptor/Akt-independent, AMPK-dependent pathway

dc.creatorOliveira, RLGS
dc.creatorUeno, M
dc.creatorde Souza, CT
dc.creatorPereira-da-Silva, M
dc.creatorGasparetti, AL
dc.creatorBezzera, RMN
dc.creatorAlberici, LC
dc.creatorVercesi, AE
dc.creatorSaad, MJA
dc.creatorVelloso, LA
dc.date2004
dc.dateOCT
dc.date2014-11-15T12:40:11Z
dc.date2015-11-26T16:11:16Z
dc.date2014-11-15T12:40:11Z
dc.date2015-11-26T16:11:16Z
dc.date.accessioned2018-03-28T22:59:45Z
dc.date.available2018-03-28T22:59:45Z
dc.identifierAmerican Journal Of Physiology-endocrinology And Metabolism. Amer Physiological Soc, v. 287, n. 4, n. E686, n. E695, 2004.
dc.identifier0193-1849
dc.identifierWOS:000223791400013
dc.identifier10.1152/ajpendo.00103.2004
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/78926
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/78926
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/78926
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1267044
dc.descriptionPeroxisome proliferator-activated receptor-gamma coactivator-1alpha (PGC-1alpha) participates in control of expression of genes involved in adaptive thermogenesis, muscle fiber type differentiation, and fuel homeostasis. The objective of the present study was to evaluate the participation of cold-induced PGC-1alpha expression in muscle fiber type-specific activity of proteins that belong to the insulin-signaling pathway. Rats were exposed to 4degreesC for 4 days and acutely treated with insulin in the presence or absence of an antisense oligonucleotide to PGC-1alpha. Cold exposure promoted a significant increase of PGC-1alpha and uncoupling protein-3 protein expression in type I and type II fibers of gastrocnemius muscle. In addition, cold exposure led to higher glucose uptake during a hyperinsulinemic clamp, which was accompanied by higher expression and membrane localization of GLUT4 in both muscle fiber types. Cold exposure promoted significantly lower insulin-induced tyrosine phosphorylation of the insulin receptor (IR) and Ser(473) phosphorylation of acute transforming retrovirus thymoma (Akt) and an insulin-independent increase of Thr(172) phosphorylation of adenosine 5'-monophosphate-activated protein kinase (AMPK). Inhibition of PGC-1alpha expression in cold-exposed rats by antisense oligonucleotide treatment diminished glucose clearance rates during a hyperinsulinemic clamp and reduced expression and membrane localization of GLUT4. Reduction of PGC-1alpha expression resulted in no modification of insulin-induced tyrosine phosphorylation of the IR and Ser(473) phosphorylation of Akt. Finally, reduction of PGC-1alpha resulted in lower Thr(172) phosphorylation of AMPK. Thus cold-induced hyperexpression of PGC-1alpha participates in control of skeletal muscle glucose uptake through a mechanism that controls GLUT4 expression and subcellular localization independent of the IR and Akt activities but dependent on AMPK.
dc.descriptiono TEXTO COMPLETO DESTE ARTIGO, ESTARÁ DISPONÍVEL À PARTIR DE AGOSTO DE 2015.
dc.description287
dc.description4
dc.descriptionE686
dc.descriptionE695
dc.languageen
dc.publisherAmer Physiological Soc
dc.publisherBethesda
dc.publisherEUA
dc.relationAmerican Journal Of Physiology-endocrinology And Metabolism
dc.relationAm. J. Physiol.-Endocrinol. Metab.
dc.rightsembargo
dc.sourceWeb of Science
dc.subjectacute transforming retrovirus thymoma
dc.subjectadenosine 5 '-monophosphate-activated protein kinase
dc.subjectcold exposure
dc.subjectActivated Protein-kinase
dc.subjectTranscriptional Coactivator Pgc-1
dc.subjectSkeletal-muscle
dc.subjectUncoupling Protein-3
dc.subjectGamma Coactivator-1
dc.subjectTyrosine Kinase
dc.subjectEnzyme-activity
dc.subjectMessenger-rna
dc.subjectResistance
dc.subjectPhosphorylation
dc.titleCold-induced PGC-1 alpha expression modulates muscle glucose uptake through an insulin receptor/Akt-independent, AMPK-dependent pathway
dc.typeArtículos de revistas


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