Combination of angiotensin-converting enzyme and methylenetetrahydrofolate reductase gene polymorphisms as determinant risk factors for chronic allograft dysfunction

dc.creatorde Alvarenga, MPS
dc.creatorPavarino-Bertelli, EC
dc.creatorAbbud, M
dc.creatorFerreira-Baptista, MAS
dc.creatorHaddad, R
dc.creatorEberlin, MN
dc.creatorGoloni-Bertollo, EM
dc.date2007
dc.dateJAN-FEB
dc.date2014-11-15T11:56:03Z
dc.date2015-11-26T16:10:57Z
dc.date2014-11-15T11:56:03Z
dc.date2015-11-26T16:10:57Z
dc.date.accessioned2018-03-28T22:59:35Z
dc.date.available2018-03-28T22:59:35Z
dc.identifierTransplantation Proceedings. Elsevier Science Inc, v. 39, n. 1, n. 78, n. 80, 2007.
dc.identifier0041-1345
dc.identifierWOS:000244089100015
dc.identifier10.1016/j.transproceed.2006.10.224
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/79000
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/79000
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/79000
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1267003
dc.descriptionObjective. The aim of this study was to investigate the frequency of gene angiotensincoVerting enzyme insertion/deletion (ACE I/D) and methylenetetrahydrofolate reductase (MTHFR C677T and A1298C) variants, as well as to evaluate the plasma homocysteine concentrations in 217 patients who underwent renal transplantation at least 12 months prior to define risk factors for chronic allograft dysfunction. Methods. The presence of the polymorphism ACE deletion was assessed by polymerase chain reaction (PCR) analysis. MTHFR polymorphisms were determined by PCR and restriction fragment length polymorphism (RFPL) techniques. The restriction enzymes were Hinf I and Mbo II for MTHFR variants C677T and A1298C, respectively. Plasma homocysteine concentrations were measured by liquid chromatography-tandem mass spectrometry (LS-MS/MS). Results. Hyperhomocysteinernias were more common in patients with chronic allograft dysfunction (P =.004). No statistically significant differences were observed between the allelic and genotypic distributions of MTHFR and ACE polymorphisms. An effective risk factor was found when the polymorphisms of the ACE and MTHFR genes and hyperhomocysteinemia were associated (odds ratio 2.51; 95% confidence interval 1.19-5.28). In conclusion, our study identified that the presence of hyperhomocysteinernia in combination with unfavorable genotypes contributes to an increased risk for development of chronic allograft dysfunction.
dc.description39
dc.description1
dc.description78
dc.description80
dc.languageen
dc.publisherElsevier Science Inc
dc.publisherNew York
dc.publisherEUA
dc.relationTransplantation Proceedings
dc.relationTransplant. Proc.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectRenal-transplant Recipients
dc.subjectTotal Homocysteine
dc.subjectNephropathy
dc.subjectPlasma
dc.subjectMthfr
dc.subjectHyperhomocysteinemia
dc.subjectVitamin-b-12
dc.subjectCysteine
dc.subjectDisease
dc.subjectFolate
dc.titleCombination of angiotensin-converting enzyme and methylenetetrahydrofolate reductase gene polymorphisms as determinant risk factors for chronic allograft dysfunction
dc.typeArtículos de revistas


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