Apoptosis of spinal interneurons induced by sciatic nerve axotomy in the neonatal rat is counteracted by nerve growth factor and ciliary neurotrophic factor

dc.creatorOliveira, ALR
dc.creatorRisling, M
dc.creatorNegro, A
dc.creatorLangone, F
dc.creatorCullheim, S
dc.date2002
dc.date40330
dc.date2014-11-15T07:44:06Z
dc.date2015-11-26T16:09:57Z
dc.date2014-11-15T07:44:06Z
dc.date2015-11-26T16:09:57Z
dc.date.accessioned2018-03-28T22:58:33Z
dc.date.available2018-03-28T22:58:33Z
dc.identifierJournal Of Comparative Neurology. Wiley-liss, v. 447, n. 4, n. 381, n. 393, 2002.
dc.identifier0021-9967
dc.identifierWOS:000175405100006
dc.identifier10.1002/cne.10248
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/78034
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/78034
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/78034
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1266747
dc.descriptionWe have previously shown that not only motoneurons and dorsal root ganglion cells but also small neurons, presumably interneurons in the spinal cord, may undergo apoptotic cell death as a result of neonatal peripheral nerve transection in the rat. With the aid of electron microscopy, we have here demonstrated that apoptosis in the spinal cord is confined to neurons and does not involve glial cells at the survival time studied (24 hours). To define the relative importance of the loss of a potential target (motoneuron) and a potential afferent input (dorsal root ganglion cell) for the induction of apoptosis in interneurons in this situation, we have compared the distributions and time courses for TUNEL labeling, which detects apoptotic cell nuclei, in the L5 segment of the spinal cord and the L5 dorsal root ganglion after sciatic nerve transection in the neonatal (P2) rat. In additional experiments, we studied the effects on TUNEL labeling of interneurons after treatment of the cut sciatic nerve with either ciliary neurotrophic factor (CNTF) to rescue motoneurons or nerve growth factor (NGF) to rescue dorsal root ganglion cells. The time courses of the TUNEL labeling in motoneurons and interneurons induced by the lesion show great similarities (peak at 8-48 hours postoperatively), whereas the labeling in dorsal root ganglion cells occurs later (24-72 hours). Both CNTF and NGF decrease the number of TUNEL-labeled interneurons, but there is a regional difference, in that CNTF preferentially saves interneurons in deep dorsal and ventral parts of the spinal cord, whereas the rescuing effects of NGF are seen mainly in the superficial dorsal horn. The results are interpreted as signs of a trophic dependence on both the target and the afferent input for the survival of interneurons neonatally. (C) 2002 Wiley-Liss, Inc.
dc.description447
dc.description4
dc.description381
dc.description393
dc.languageen
dc.publisherWiley-liss
dc.publisherNew York
dc.publisherEUA
dc.relationJournal Of Comparative Neurology
dc.relationJ. Comp. Neurol.
dc.rightsfechado
dc.rightshttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.sourceWeb of Science
dc.subjectTUNEL labeling
dc.subjectmotoneuron
dc.subjectdorsal root ganglion
dc.subjectnerve injury
dc.subjecttrophism
dc.subjectProgrammed Cell-death
dc.subjectDorsal-root Ganglion
dc.subjectPrimary Sensory Neurons
dc.subjectMotoneurons In-vivo
dc.subjectSubstance-p
dc.subjectPostnatal-development
dc.subjectFactor Prevents
dc.subjectMotor Neurons
dc.subjectTime-course
dc.subjectSurvival
dc.titleApoptosis of spinal interneurons induced by sciatic nerve axotomy in the neonatal rat is counteracted by nerve growth factor and ciliary neurotrophic factor
dc.typeArtículos de revistas


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