Molecular characterization of Brazilian avian pneumovirus isolates using reverse transcription-polymerase chain reaction, restriction endonuclease analysis and sequencing of a G gene fragment

dc.creatorDani, MAC
dc.creatorArns, CW
dc.creatorDurigon, EL
dc.date1999
dc.dateOCT
dc.date2014-12-02T16:24:41Z
dc.date2015-11-26T16:09:34Z
dc.date2014-12-02T16:24:41Z
dc.date2015-11-26T16:09:34Z
dc.date.accessioned2018-03-28T22:58:09Z
dc.date.available2018-03-28T22:58:09Z
dc.identifierAvian Pathology. Carfax Publishing, v. 28, n. 5, n. 473, n. 476, 1999.
dc.identifier0307-9457
dc.identifierWOS:000082972800007
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/57736
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/57736
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/57736
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1266652
dc.descriptionA reverse transcriptase-polymerase chain reaction (RT-PCR) assay was employed to amplify a G gene transcript of the avian pneumovirus (APV) in two European turkey isolates and two Brazilian chicken isolates. The PCR products were digested using restriction endonucleases and the restriction patterns were compared with earlier reported isolate patterns. The same PCR products were cloned into pUC18 and sequenced. Restriction patterns and sequence analyses of the approximately 600 bp products from both Brazilian isolates revealed 99% identity with earlier reported subgroup A APV sequence, suggesting that these isolates belong to this subgroup.
dc.description28
dc.description5
dc.description473
dc.description476
dc.languageen
dc.publisherCarfax Publishing
dc.publisherBasingstoke
dc.publisherInglaterra
dc.relationAvian Pathology
dc.relationAvian Pathol.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectSwollen Head Syndrome
dc.subjectRhinotracheitis
dc.subjectChickens
dc.subjectAntibodies
dc.subjectViruses
dc.subjectTrt
dc.titleMolecular characterization of Brazilian avian pneumovirus isolates using reverse transcription-polymerase chain reaction, restriction endonuclease analysis and sequencing of a G gene fragment
dc.typeArtículos de revistas


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