Co-expression and regulation of connexins 36 and 43 in cultured neonatal rat pancreatic islets

dc.creatorLeite, AR
dc.creatorCarvalho, CPF
dc.creatorFurtado, AG
dc.creatorBarbosa, HCL
dc.creatorBoschero, AC
dc.creatorCollares-Buzato, CB
dc.date2005
dc.dateFEB
dc.date2014-11-15T04:17:38Z
dc.date2015-11-26T16:09:26Z
dc.date2014-11-15T04:17:38Z
dc.date2015-11-26T16:09:26Z
dc.date.accessioned2018-03-28T22:58:01Z
dc.date.available2018-03-28T22:58:01Z
dc.identifierCanadian Journal Of Physiology And Pharmacology. Natl Research Council Canada, v. 83, n. 2, n. 142, n. 151, 2005.
dc.identifier0008-4212
dc.identifierWOS:000228450100003
dc.identifier10.1139/Y04-133
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/78850
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/78850
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/78850
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1266616
dc.descriptionFetal and neonatal pancreatic islets present a lower insulin secretory response as compared with adult islets. Prolonged culturing leads to an improvement of the glucose-induced insulin secretion response in neonatal pancreatic islets that may involve regulation of gap junction mediated cell communication. In this study, we investigated the effect of culturing neonatal islet cells for varying periods of time and with different glucose medium concentrations on the cellular expression of the endocrine pancreatic gap junction associated connexin (Cx) 36 and Cx43. We report here that the 7-d culture induced upregulation of the expression of these junctional proteins in neonatal islets in a time-dependent manner. A correlation was observed between the increased mRNA and protein expression of Cx36 and Cx43 and the increased insulin secretion following islet culturing. In addition, increasing glucose concentration within the culture medium induced a concentration-dependent enhancement of Cx36 islet expression, but not of Cx43 expression in cultured neonatal islets. In conclusion, we suggest that the regulation of gap junctional proteins by culture medium containing factors and glucose may be an important event for the maturation process of P cells observed at in vitro conditions.
dc.description83
dc.description2
dc.description142
dc.description151
dc.languageen
dc.publisherNatl Research Council Canada
dc.publisherOttawa
dc.publisherCanadá
dc.relationCanadian Journal Of Physiology And Pharmacology
dc.relationCan. J. Physiol. Pharmacol.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectconnexin 36
dc.subjectconnexin 43
dc.subjectgap junctions
dc.subjectinsulin secretion
dc.subjectin vitro maturation
dc.subjectneonatal pancreatic islets
dc.subjectInduced Insulin-secretion
dc.subjectBeta-cells
dc.subjectB-cells
dc.subjectGene-expression
dc.subjectGap-junctions
dc.subjectInvivo Modulation
dc.subjectIn-vitro
dc.subjectGlucose
dc.subjectProlactin
dc.subjectProteins
dc.titleCo-expression and regulation of connexins 36 and 43 in cultured neonatal rat pancreatic islets
dc.typeArtículos de revistas


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