Alterations in the permeability of dystrophic fibers during neuromuscular junction development

dc.creatorMarques, MJ
dc.creatorMatsumura, CY
dc.creatorSanto, H
dc.date2007
dc.dateMAR
dc.date2014-11-14T23:25:20Z
dc.date2015-11-26T16:09:01Z
dc.date2014-11-14T23:25:20Z
dc.date2015-11-26T16:09:01Z
dc.date.accessioned2018-03-28T22:57:36Z
dc.date.available2018-03-28T22:57:36Z
dc.identifierActa Biologica Hungarica. Akademiai Kiado, v. 58, n. 1, n. 1, n. 9, 2007.
dc.identifier0236-5383
dc.identifierWOS:000245012100001
dc.identifier10.1556/ABiol.58.2007.1.1
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/77189
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/77189
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/77189
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1266512
dc.descriptionIn the mdx mice, lack of dystrophin leads to increases in calcium influx and myonecrosis, followed by muscle regeneration. Synapse elimination is faster in mdx than in controls, suggesting that increases in calcium influx during development could be involved. In the present study, we evaluated whether dystrophic fibers display changes in permeability to Evans Blue Dye (EBD) during development of the neuromuscular junction. EBD is a sensitive label for the early detection of increased myofiber permeability and sarcolemmal damage. After intraperitoneal injection of EBD, sternomastoid (STN) and tibialis anterior (T. anterior) muscles were analyzed with fluorescence microscopy. At 01, 07 and 14 days of age, STN and TA mdx myofibers were not stained with EBD. At 21 days of age, positive labeling of TA and STN mdx myofibers was seen, suggesting permeability modification and myonecrosis. Adult Muscles showed a decrease (T. anterior) or no changes (STN) in the amount of EBD-positive fibers. These results suggest that there is no sarcolemmal damage detected by EBD during development of dystrophic neuromuscular junctions and other factors may contribute to the earlier synapse elimination seen in dystrophic muscle.
dc.description58
dc.description1
dc.description1
dc.description9
dc.languageen
dc.publisherAkademiai Kiado
dc.publisherBudapest
dc.publisherHungria
dc.relationActa Biologica Hungarica
dc.relationActa Biol. Hung.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectcalcium regulation
dc.subjectdystrophin
dc.subjectEvans Blue
dc.subjectmdx
dc.subjectneuromuscular junction
dc.subjectsynapse elimination
dc.subjectDuchenne Muscular-dystrophy
dc.subjectNitric-oxide Synthase
dc.subjectMdx Mice
dc.subjectSkeletal-muscle
dc.subjectEvans Blue
dc.subjectMouse
dc.subjectDegeneration
dc.subjectExercise
dc.subjectTime
dc.titleAlterations in the permeability of dystrophic fibers during neuromuscular junction development
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución