Nucleus image properties assessed by video image analysis in mouse hepatocytes under a short lysis for extended chromatin fiber formation

dc.creatorVidal, BC
dc.creatorMoraes, AS
dc.creatorMello, MLS
dc.date2006
dc.date37196
dc.date2014-11-14T23:33:18Z
dc.date2015-11-26T16:08:54Z
dc.date2014-11-14T23:33:18Z
dc.date2015-11-26T16:08:54Z
dc.date.accessioned2018-03-28T22:57:30Z
dc.date.available2018-03-28T22:57:30Z
dc.identifierCytometry Part A. Wiley-liss, v. 69A, n. 11, n. 1106, n. 1113, 2006.
dc.identifier1552-4922
dc.identifierWOS:000241685900002
dc.identifier10.1002/cyto.a.20339
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/62201
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/62201
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/62201
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1266483
dc.descriptionBackground: How much DNA remains in mouse hepatocyte nuclei after extended chromatin fiber (ECF) formation or whether this content varies within the nuclear population is not known. This information could be relevant to understanding chromatin extensibility as related to chromatin organization, possibly associated with variable nuclear activities in hepatocytes. Methods: A protocol for ECF formation under the gravity action, image analysis of Feulgen-stained unfixed mouse hepatocyte remnants, and DAPI fluorescence were used. Results: Areas, shape, Feulgen-DNA amounts, and chromatin texture were affected in unfixed, lysed nuclei. The Feulgen-DNA values in nuclear remnants represented similar to 37% of the content in fixed, nonlysed nuclei in terms of median values; the coefficient of variation of Feulgen-DNA values in the nuclear remnants was much higher than those in controls. Enhancement in DAPI fluorescence was evident in chromocenters of the fixed nuclei and in remnants and some ECF granules of the unfixed, lysed nuclei. Conclusions: The DNA content of the nuclear remnants was much more variable than that assumed from known variability in hepatocyte ploidy degrees. The variable constraint to chromatin extrusion from hepatocyte nuclei is, hypothesized to depend on variable chromatin organization with possible involvement of nuclear matrix association, transcriptional, activities, and AT-rich DNA-containing heterochromatin. (c) 2006 International Society for Analytical Cytology.
dc.description69A
dc.description11
dc.description1106
dc.description1113
dc.languageen
dc.publisherWiley-liss
dc.publisherHoboken
dc.publisherEUA
dc.relationCytometry Part A
dc.relationCytom. Part A
dc.rightsfechado
dc.rightshttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.sourceWeb of Science
dc.subjectextended chromatin fibers
dc.subjectchromatin supraorganization
dc.subjectnuclear remnants
dc.subjectFeulgen-DNA amount-hepatocytes
dc.subjectIn-situ Hybridization
dc.subjectCell Polyploidy
dc.subjectSatellite Dna
dc.subjectLiver
dc.subjectHeterochromatin
dc.subjectGrowth
dc.subjectChromosomes
dc.subjectProteins
dc.titleNucleus image properties assessed by video image analysis in mouse hepatocytes under a short lysis for extended chromatin fiber formation
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución