Image analysis of the AgNOR response in ras-transformed human breast epithelial cells

dc.creatorMello, MLS
dc.creatorVidal, BC
dc.creatorRusso, J
dc.creatorPlanding, W
dc.creatorSchenck, U
dc.date2008
dc.date2014-11-14T17:18:29Z
dc.date2015-11-26T16:07:37Z
dc.date2014-11-14T17:18:29Z
dc.date2015-11-26T16:07:37Z
dc.date.accessioned2018-03-28T22:56:18Z
dc.date.available2018-03-28T22:56:18Z
dc.identifierActa Histochemica. Elsevier Gmbh, Urban & Fischer Verlag, v. 110, n. 3, n. 210, n. 216, 2008.
dc.identifier0065-1281
dc.identifierWOS:000256420300005
dc.identifier10.1016/j.acthis.2007.10.016
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/69223
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/69223
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/69223
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1266179
dc.descriptionThe argyrophylic staining of the nucleolar organizer regions (AgNOR positive response) in interphase nuclei is often related directly to the cellular demand for ribosome biogenesis and is considered of relevance in studies of tumor pathology. Transformation of human breast epithelial MCF-10A cells by the c-Ha-ras oncogene results in altered growth, invasiveness and tumorigenicity in nude mice. Since ras transformation may be associated with a more intense nucleolar activity, we examined the influence of transfection by the Ha-ras oncogene on AgNOR staining response in MCF-10A cells. Following assessment of the AgNOR response with video image analysis, the AgNOR-positive areas and the AgNOR area/nuclear area ratio, but not the number of AgNOR aggregates or dots per nucleus, were found to be much higher after ras transformation. A role of the Ha-ras transformation on the nucleolar activity of the MCF-10A is thus suggested as assessed by the AgNOR staining. Based on data in the literature, it is also hypothesized that a decreased wild-type p53 level, possibly promoted by the ras transformation, may be associated with the increased AgNOR response. (c) 2007 Elsevier GmbH. All rights reserved.
dc.description110
dc.description3
dc.description210
dc.description216
dc.languageen
dc.publisherElsevier Gmbh, Urban & Fischer Verlag
dc.publisherJena
dc.publisherAlemanha
dc.relationActa Histochemica
dc.relationActa Histochem.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectAgNOR
dc.subjectHa-ras oncogene
dc.subjecthuman breast epithelial cells
dc.subjectin vitro
dc.subjectp53
dc.subjectimage analysis
dc.subjectCancer Specimens
dc.subjectPrognostic Value
dc.subjectLiver-cells
dc.subjectP53 Status
dc.subjectNuclear
dc.subjectExpression
dc.subjectOncogene
dc.subjectCarcinoma
dc.subjectCytometry
dc.subjectHistology
dc.titleImage analysis of the AgNOR response in ras-transformed human breast epithelial cells
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución