HRP-based amperometric biosensor for the polyphenols determination in vegetables extract

dc.creatorMello, LD
dc.creatorSotomayor, MDPT
dc.creatorKubota, LT
dc.date2003
dc.dateDEC 1
dc.date2014-11-14T14:43:21Z
dc.date2015-11-26T16:07:01Z
dc.date2014-11-14T14:43:21Z
dc.date2015-11-26T16:07:01Z
dc.date.accessioned2018-03-28T22:55:48Z
dc.date.available2018-03-28T22:55:48Z
dc.identifierSensors And Actuators B-chemical. Elsevier Science Sa, v. 96, n. 3, n. 636, n. 645, 2003.
dc.identifier0925-4005
dc.identifierWOS:000187039100024
dc.identifier10.1016/j.snb.2003.07.008
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/68887
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/68887
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/68887
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1266055
dc.descriptionThe determination of polyphenols is important due to their biological importance as plant secondary metabolite, their ecological role in the antioxidant capacity, physiological effects, employment as markers in taxonomic studies, and their properties to food quality control. Therefore, a biosensor based on horseradish peroxidase (HRP) and DNA immobilized onto silica-titanium is presented, and its application for measuring the polyphenols compounds in vegetables samples was studied. Various analytical parameters influencing the biosensor performance have been studied as function of the chlorogenic acid (CGA) polyphenol compound, parameters such as working potential, type and concentration of the buffer, pH, response time and response with other compounds were investigated. In the optimized conditions, the biosensor presented a linear response range for CGA from 1 to 50 mumol l(-1), applying a potential of -50 mV versus Ag/AgCl, with a sensitivity of 181 mumol(-1) 1 nA cm(-2) and a detection limit of 0.7 mumol l(-1). The biosensor was tested in the polyphenols determination in vegetables extract and the results were compared with the Folin-Ciocalteau traditional method and the biosensor showed suitability to the quantification of the total polyphenol in the tested samples. (C) 2003 Elsevier B.V. All rights reserved.
dc.description96
dc.description3
dc.description636
dc.description645
dc.languageen
dc.publisherElsevier Science Sa
dc.publisherLausanne
dc.publisherSuíça
dc.relationSensors And Actuators B-chemical
dc.relationSens. Actuator B-Chem.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectbiosensor
dc.subjecthorseradish peroxidase
dc.subjectpolyphenols
dc.subjectfood analysis
dc.subjectPhenolic-compounds
dc.subjectOlive Oils
dc.subjectRancidification Process
dc.subjectHorseradish-peroxidase
dc.subjectNatural Polyphenols
dc.subjectHydrogen-peroxide
dc.subjectAntioxidants
dc.subjectElectrodes
dc.subjectSilica
dc.subjectChemistry
dc.titleHRP-based amperometric biosensor for the polyphenols determination in vegetables extract
dc.typeArtículos de revistas


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