Prognostic markers of high-grade squamous intraepithelial lesions: the role of p16(INK4a) and high-risk human papillomavirus

dc.creatorEleuterio, J
dc.creatorGiraldo, PC
dc.creatorGoncalves, AK
dc.creatorCavalcante, DIM
dc.creatorFerreira, FVD
dc.creatorMesquita, SM
dc.creatorMorais, SS
dc.date2007
dc.date2014-11-14T12:45:54Z
dc.date2015-11-26T16:06:36Z
dc.date2014-11-14T12:45:54Z
dc.date2015-11-26T16:06:36Z
dc.date.accessioned2018-03-28T22:55:24Z
dc.date.available2018-03-28T22:55:24Z
dc.identifierActa Obstetricia Et Gynecologica Scandinavica. Taylor & Francis As, v. 86, n. 1, n. 94, n. 98, 2007.
dc.identifier0001-6349
dc.identifierWOS:000246475900016
dc.identifier10.1080/00016340601089727
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/70486
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/70486
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/70486
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1265955
dc.descriptionBackground. p16(INK4a) seems to be an indicator of the grade of Human Papillomavirus-induced lesions and a possible predictor of the lesion evolution. There are few studies about the role of HPV test and p16(INK4a) in diagnosis of high-grade cervical lesions in South-American women. The aim of the present study was to evaluate the presence of p16(INK4a) and high-risk HPV-DNA expression in cases diagnosed as squamous intra-epithelial lesion and evaluate their role in the approach of high-grade squamous intra-epithelial lesion. Methods. p16(INK4a) and high-risk Human papillomavirus were investigated in 96 samples of the cervix ( 13 cases of high grade squamous intraepithelial lesions, 26 cases of low grade intraepithelial lesions and 57 normal tissues). The p16(INK4a) was identified by immunohistochemistry using the p16(INK4a) kit (E6H4 clone, DakoCytomation, Carpinteria, CA) and Human papillomavirus DNA was classified by hybrid capture (Digene (R)). Associations were evaluated by the KAPPA index. Results. The p16(INK4a) was detected in 92.3% of the high-grade squamous intraepithelial lesions, in 15.4% of the low-grade and in none of the normal tissues. The sensitivity, specificity, positive predictive value and negative predictive value for high-grade lesion were 92.3%, 100%, 100%, and 98.3%, respectively when considering p16(INK4a) expression, and 100%, 70.2%, 43.3% and 100%, respectively when considering high-risk HPV. Conclusions. p16(INK4a) test was better associated with high-grade intraepithelial lesion ( kappa = 0.95) than was the presence of high-risk HPV ( kappa = 0.47). Both tests could be complementary to high-grade squamous intra-epithelial lesion screening and help to define the diagnosis of the inconclusive low-grade/high-grade squamous intraepithelial lesion cases.
dc.description86
dc.description1
dc.description94
dc.description98
dc.languageen
dc.publisherTaylor & Francis As
dc.publisherOslo
dc.publisherNoruega
dc.relationActa Obstetricia Et Gynecologica Scandinavica
dc.relationActa Obstet. Gynecol. Scand.
dc.rightsfechado
dc.rightshttp://journalauthors.tandf.co.uk/permissions/reusingOwnWork.asp
dc.sourceWeb of Science
dc.subjectsquamous intraepithelial lesion
dc.subjecthuman papillomavirus
dc.subjecttumor markers
dc.subjectp16(INK4a)
dc.subjectExpression
dc.subjectNeoplasia
dc.subjectProtein
dc.subjectProgression
dc.subjectCin
dc.subjectCervix
dc.subjectP16
dc.subjectP53
dc.titlePrognostic markers of high-grade squamous intraepithelial lesions: the role of p16(INK4a) and high-risk human papillomavirus
dc.typeArtículos de revistas


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