Adsorption of salivary and serum proteins, and bacterial adherence on titanium and zirconia ceramic surfaces

dc.creatorLima, EMCX
dc.creatorKoo, H
dc.creatorSmith, AMV
dc.creatorRosalen, PL
dc.creatorCury, AADB
dc.date2008
dc.dateAUG
dc.date2014-11-14T11:36:41Z
dc.date2015-11-26T16:06:21Z
dc.date2014-11-14T11:36:41Z
dc.date2015-11-26T16:06:21Z
dc.date.accessioned2018-03-28T22:55:10Z
dc.date.available2018-03-28T22:55:10Z
dc.identifierClinical Oral Implants Research. Wiley-blackwell, v. 19, n. 8, n. 780, n. 785, 2008.
dc.identifier0905-7161
dc.identifierWOS:000257514000008
dc.identifier10.1111/j.1600-0501.2008.01524.x
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/76911
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/76911
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/76911
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1265893
dc.descriptionObjectives: The aim of this study was to determine the pattern of salivary and serum proteins present in pellicles formed on titanium (Ti) and zirconia ceramic (ZrO(2)) surfaces, and the ability of bacterial cells to adhere to the experimental pellicles. In addition, the protein profiles and bacterial binding properties of pellicles on Ti and ZrO(2) were compared to those formed on hydroxyapatite (HA) surface. Methods: The pellicles were formed in vitro by incubating the materials with whole saliva, serum or saliva+serum. Protein composition in each of the pellicles was investigated by SDS-PAGE and immunodetection. The adherence of radiolabeled Streptococcus mutans and Actinomyces naeslundii to uncoated surfaces and experimental pellicles was determined by means of scintillation counting. Statistical analyses were done using ANOVA and Tukey's test at significance level at P < 0.05. In general, the electrophoretic analysis of the pellicles formed on HA, Ti and ZrO(2) revealed few qualitative differences of the composition of proteins of the pellicles formed on HA, Ti and ZrO(2) surfaces. Pellicle components identified included amylase, IgA, IgG, albumin, fibronectin and fibrinogen. The number of S. mutans cells adhered to uncoated Ti and ZrO(2) was significantly higher than those adhered to HA (P < 0.05). In contrast, lower number of A. naeslundii cells adhered to uncoated Ti and ZrO(2) than to HA (P < 0.05). However, the presence of saliva and saliva+serum pellicles greatly reduced the number of S. mutans cells bound to each of the surfaces. The data showed that Ti and ZrO(2) display similar pellicle protein composition and bacterial binding properties; however, significant differences were observed when both materials were compared to HA.
dc.description19
dc.description8
dc.description780
dc.description785
dc.languageen
dc.publisherWiley-blackwell
dc.publisherMalden
dc.publisherEUA
dc.relationClinical Oral Implants Research
dc.relationClin. Oral Implant. Res.
dc.rightsfechado
dc.rightshttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.sourceWeb of Science
dc.subjectbacterial adherence
dc.subjectsalivary pellicle
dc.subjectserum proteins
dc.subjecttitanium
dc.subjectzirconia
dc.subjectIn-vitro
dc.subjectCoated Hydroxyapatite
dc.subjectStreptococcus-mutans
dc.subjectOral Streptococci
dc.subjectAlpha-amylase
dc.subjectPolyacrylamide Gels
dc.subjectPlaque-formation
dc.subjectDental Plaque
dc.subjectWhole Saliva
dc.subjectPellicles
dc.titleAdsorption of salivary and serum proteins, and bacterial adherence on titanium and zirconia ceramic surfaces
dc.typeArtículos de revistas


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