Hydrogel protection: a novel approach to reduce bowel inflammation in experimental gastroschisis

dc.creatorGoncalves, FLL
dc.creatorda Silva, R
dc.creatorSchmidt, AF
dc.creatorde Oliveira, MG
dc.creatorSbragia, L
dc.date2010
dc.dateJAN
dc.date2014-11-14T08:44:36Z
dc.date2015-11-26T16:05:32Z
dc.date2014-11-14T08:44:36Z
dc.date2015-11-26T16:05:32Z
dc.date.accessioned2018-03-28T22:54:31Z
dc.date.available2018-03-28T22:54:31Z
dc.identifierEuropean Journal Of Obstetrics & Gynecology And Reproductive Biology. Elsevier Science Bv, v. 148, n. 1, n. 35, n. 39, 2010.
dc.identifier0301-2115
dc.identifierWOS:000274103500007
dc.identifier10.1016/j.ejogrb.2009.10.009
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/68982
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/68982
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/68982
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1265729
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionObjective: In gastroschisis there is herniation of the fetal bowel into the amniotic cavity that results in severe intestinal dysfunction. In order to reduce bowel exposure to amniotic fluid we used a hydrogel of N-isopropylacrylamide copolymerized with acrylic acid (P(NIPAAm-co-AAc)) to coat the herniated bowel through the use of a fibrin adhesive (Beriplast(R)). Study design: Gastroschisis was created in fetuses of 31 pregnant Sprague-Dawley rats by evisceration of the bowel through a right paramedian incision in the abdominal wall on day 18.5 of pregnancy. The fetuses were separated in four groups of 12 fetuses: control (C), gastroschisis (G), gastroschisis + fibrin adhesive (GA) and gastroschisis + fibrin adhesive + dry hydrogel (GAH). Animals were harvested at day 21.5 of pregnancy and the hydrogel was removed. Fetuses and bowels were weighed and morphometric analysis was performed. Isoelectric focusing of the amniotic fluid determined its electrical charge. We evaluated the hydrogel swelling ratio (Q) in the amniotic fluid. Histological analysis and scanning electronic microscopy (SEM) of the bowel and hydrogel were performed. Our primary outcome was bowel intactness after hydrogel removal and our secondary outcome was the effectiveness of the hydrogel in protecting the bowel against amniotic fluid and its components. Differences among the groups were tested by the ANOVA and Tukey-Kramer post-test method and the statistical significance accepted was for p values <0.05. Results: The mass of swollen hydrogel was 34 times the mass of dry hydrogel. Isoelectric focusing of the amniotic fluid showed that most of its proteins are negatively charged as the hydrogel. SEM showed that removal of the hydrogel did not damage bowel serosa. Bowel weight, diameter and wall thickness were similar between groups C and GAH but bowel diameter and wall thickness was significantly reduced in C and GAH compared to G and GA (p < 0.001). Conclusion: The P(NIPAAm-co-AAc) hydrogel does not harm the bowel and provides a safe effective protection with reduction of bowel damage in gastroschisis. (C) 2009 Elsevier Ireland Ltd. All rights reserved.
dc.description148
dc.description1
dc.description35
dc.description39
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFAPESP [04/11204-4, 08/51487-9]
dc.languageen
dc.publisherElsevier Science Bv
dc.publisherAmsterdam
dc.publisherHolanda
dc.relationEuropean Journal Of Obstetrics & Gynecology And Reproductive Biology
dc.relationEur. J. Obstet. Gynecol. Reprod. Biol.
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectHydrogel
dc.subjectBiomaterial
dc.subjectGastroschisis
dc.subjectScanning microscopy
dc.subjectInflammation
dc.subjectBiocompatibility
dc.subjectFetal-rat Model
dc.subjectIntestinal Damage
dc.subjectMorphology
dc.subjectEtiology
dc.subjectRepair
dc.subjectFluid
dc.titleHydrogel protection: a novel approach to reduce bowel inflammation in experimental gastroschisis
dc.typeArtículos de revistas


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