Oxidation of LDL enhances the cholesteryl ester transfer protein (CETP)-mediated cholesteryl ester transfer rate to HDL, bringing on a diminished net transfer of cholesteryl ester from HDL to oxidized LDL

dc.creatorCastilho, LN
dc.creatorOliveira, HCF
dc.creatorCazita, PM
dc.creatorde Oliveira, AC
dc.creatorSesso, A
dc.creatorQuintao, ECR
dc.date2001
dc.dateFEB
dc.date2014-11-14T03:25:57Z
dc.date2015-11-26T16:04:16Z
dc.date2014-11-14T03:25:57Z
dc.date2015-11-26T16:04:16Z
dc.date.accessioned2018-03-28T22:53:21Z
dc.date.available2018-03-28T22:53:21Z
dc.identifierClinica Chimica Acta. Elsevier Science Bv, v. 304, n. 41671, n. 99, n. 106, 2001.
dc.identifier0009-8981
dc.identifierWOS:000167221700012
dc.identifier10.1016/S0009-8981(00)00401-0
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/81567
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/81567
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/81567
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1265439
dc.descriptionCholesteryl ester transfer protein (CETP) plays a controversial role in atherogenesis by contributing to the net transfer of high density lipoprotein (HDL) cholesteryl ester (CE) to the liver via apolipoprotein-B-containing lipoproteins (apoB-LP). We evaluated in vitro the CETP-mediated bidirectional transfer of CE from HDL to the chemically modified pro-atherogenic low density lipoprotein (LDL) particles. Acetylated or oxidized (ox) LDL. either unlabeled or [H-3]-CE labeled. were incubated with [C-14]-CE-HDL in the presence of the lipoprotein-deficient plasma fraction (d>1.21 g/ml) as the source of CETP. The amount of radioactive CE transferred was determined after dextran sulfate/MgCl2, precipitation of LDL. The results showed a 1.4-2.8-fold lower HDL-CE transfer to acetylated LDL while no effect was: observed on the CE transfer to oxidized LDL. However. the reverse transfer rate of [H-3]CE-LDL to HDL was 1.4-3.6 times greater when LDL was oxidized than when it was intact. Overall, HDL, was better than HDL, as donor of CE to native LDL. probably reflecting the relatively greater CE content of HDL2. Oxidation of LDL enhanced the CETP-mediated cholesteryl ester transfer rate to HDL, bringing on a reduced net transfer rate of cholesteryl ester from HDL to ox LDL. This may diminish the oxLDL particle's atherogenic effect. (C) 2001 Elsevier Science B.V. All rights reserved.
dc.description304
dc.description41671
dc.description99
dc.description106
dc.languageen
dc.publisherElsevier Science Bv
dc.publisherAmsterdam
dc.publisherHolanda
dc.relationClinica Chimica Acta
dc.relationClin. Chim. Acta
dc.rightsfechado
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.sourceWeb of Science
dc.subjectoxidized LDL
dc.subjectacetylated LDL
dc.subjectcholesteryl ester transfer protein
dc.subjectHDL
dc.subjectreverse cholesterol transport
dc.subjectLow-density-lipoprotein
dc.subjectLipid Transfer Protein
dc.subjectTransgenic Mice
dc.subjectCombined Hyperlipidemia
dc.subjectPlasma-lipoproteins
dc.subjectDiabetes-mellitus
dc.subjectAdipose-tissue
dc.subjectMessenger-rna
dc.subjectAtherosclerosis
dc.subjectGene
dc.titleOxidation of LDL enhances the cholesteryl ester transfer protein (CETP)-mediated cholesteryl ester transfer rate to HDL, bringing on a diminished net transfer of cholesteryl ester from HDL to oxidized LDL
dc.typeArtículos de revistas


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