Overexpression of plant uncoupling mitochondrial protein in transgenic tobacco increases tolerance to oxidative stress

dc.creatorBrandalise, M
dc.creatorMaia, IG
dc.creatorBorecky, J
dc.creatorVercesi, AE
dc.creatorArruda, P
dc.date2003
dc.dateJUN
dc.date2014-11-14T03:04:20Z
dc.date2015-11-26T16:04:03Z
dc.date2014-11-14T03:04:20Z
dc.date2015-11-26T16:04:03Z
dc.date.accessioned2018-03-28T22:53:13Z
dc.date.available2018-03-28T22:53:13Z
dc.identifierJournal Of Bioenergetics And Biomembranes. Kluwer Academic/plenum Publ, v. 35, n. 3, n. 203, n. 209, 2003.
dc.identifier0145-479X
dc.identifierWOS:000183994500002
dc.identifier10.1023/A:1024603530043
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/81543
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/81543
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/81543
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1265403
dc.descriptionAn Arabidopsis thaliana cDNA clone encoding a plant uncoupling mitochondrial protein (AtPUMP1) was overexpressed in transgenic tobacco plants. Analysis of the AtPUMP1 mRNA content in the transgenic lines, determined by Northern blot, revealed variable levels of transgene expression. Antibody probing of Western blots of mitochondrial proteins from three independent transgenic lines showed significant accumulation of AtPUMP1 in this organelle. Overproduction of AtPUMP1 in transgenic tobacco plants led to a significant increase in tolerance to oxidative stress promoted by exogenous hydrogen peroxide as compared to wild-type control plants. These results provide the first biological evidence for a role of PUMP in protection of plant cells against oxidative stress damage.
dc.description35
dc.description3
dc.description203
dc.description209
dc.languageen
dc.publisherKluwer Academic/plenum Publ
dc.publisherNew York
dc.publisherEUA
dc.relationJournal Of Bioenergetics And Biomembranes
dc.relationJ. Bioenerg. Biomembr.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjecttransgenic tobacco
dc.subjectplant uncoupling protein
dc.subjectoxidative stress
dc.subjectconstitutive overexpression
dc.subjectAcid Cycling Mechanism
dc.subjectArabidopsis-thaliana
dc.subjectHeart-mitochondria
dc.subjectHydrogen-peroxide
dc.subjectRespiratory-chain
dc.subjectLow-temperature
dc.subjectTomato Fruit
dc.subjectCytochrome-c
dc.subjectSuperoxide
dc.subjectOxygen
dc.titleOverexpression of plant uncoupling mitochondrial protein in transgenic tobacco increases tolerance to oxidative stress
dc.typeArtículos de revistas


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