Superoxide release and cellular glutathione peroxidase activity in leukocytes from children with persistent asthma

dc.creatorMarcal, LE
dc.creatorRehder, J
dc.creatorNewburger, PE
dc.creatorCondino-Neto, A
dc.date2004
dc.dateNOV
dc.date2014-11-14T02:45:55Z
dc.date2015-11-26T16:03:59Z
dc.date2014-11-14T02:45:55Z
dc.date2015-11-26T16:03:59Z
dc.date.accessioned2018-03-28T22:53:11Z
dc.date.available2018-03-28T22:53:11Z
dc.identifierBrazilian Journal Of Medical And Biological Research. Assoc Bras Divulg Cientifica, v. 37, n. 11, n. 1607, n. 1613, 2004.
dc.identifier0100-879X
dc.identifierWOS:000225128700003
dc.identifier10.1590/S0100-879X2004001100003
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/74808
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/74808
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/74808
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1265391
dc.descriptionAsthma is an inflammatory condition characterized by the involvement of several mediators, including reactive oxygen species. The aim of the present study was to investigate the superoxide release and cellular glutathione peroxidase (cGPx) activity in peripheral blood granulocytes and monocytes from children and adolescents with atopic asthma. Forty-four patients were selected and classified as having intermittent or persistent asthma (mild, moderate or severe). The spontaneous or phorbol myristate acetate (PMA, 30 nM)-induced superoxide release by granulocytes and monocytes was determined at 0, 5, 15, and 25 min. cGPx activity was assayed spectrophotometrically. The spontaneous superoxide release by granulocytes from patients with mild (N = 15), moderate (N = 12) or severe (N = 6) asthma was higher at 25 min compared to healthy individuals (N = 28, P < 0.05, Duncan test). The PMA-induced superoxide release by granulocytes from patients with moderate (N = 12) or severe (N = 6) asthma was higher at 15 and 25 min compared to healthy individuals (N = 28, P < 0.05 in both times of incubation, Duncan test). The spontaneous or PMA-induced superoxide release by monocytes from asthmatic patients was similar to healthy individuals (P > 0.05 in all times of incubation, Duncan test). cGPx activity of granulocytes and monocytes from patients with persistent asthma (N = 20) was also similar to healthy individuals (N = 10, P > 0.05, Kruskal-Wallis test). We conclude that, under specific circumstances, granulocytes from children with persistent asthma present a higher respiratory burst activity compared to healthy individuals. These findings indicate a risk of oxidative stress, phagocyte auto-oxidation, and the subsequent release of intracellular toxic oxidants and enzymes, leading to additional inflammation and lung damage in asthmatic children.
dc.description37
dc.description11
dc.description1607
dc.description1613
dc.languageen
dc.publisherAssoc Bras Divulg Cientifica
dc.publisherSao Paulo
dc.publisherBrasil
dc.relationBrazilian Journal Of Medical And Biological Research
dc.relationBrazilian J. Med. Biol. Res.
dc.rightsaberto
dc.sourceWeb of Science
dc.subjectchildren
dc.subjectasthma
dc.subjectrespiratory burst
dc.subjectgranulocytes
dc.subjectmonocytes
dc.subjectinflammation
dc.subjectRespiratory Burst
dc.subjectOxidative Stress
dc.subjectHydrogen-peroxide
dc.subjectBlood Monocytes
dc.subjectNeutrophils
dc.subjectGeneration
dc.subjectEosinophils
dc.subjectRadicals
dc.subjectSputum
dc.titleSuperoxide release and cellular glutathione peroxidase activity in leukocytes from children with persistent asthma
dc.typeArtículos de revistas


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