Bioactive Polyelectrolyte Multilayers: Hyaluronic Acid Mediated B Lymphocyte Adhesion

dc.creatorVasconcellos, FC
dc.creatorSwiston, AJ
dc.creatorBeppu, MM
dc.creatorCohen, RE
dc.creatorRubner, MF
dc.date2010
dc.dateSEP
dc.date2014-11-20T02:42:03Z
dc.date2015-11-26T16:03:40Z
dc.date2014-11-20T02:42:03Z
dc.date2015-11-26T16:03:40Z
dc.date.accessioned2018-03-28T22:52:54Z
dc.date.available2018-03-28T22:52:54Z
dc.identifierBiomacromolecules. Amer Chemical Soc, v. 11, n. 9, n. 2407, n. 2414, 2010.
dc.identifier1525-7797
dc.identifierWOS:000281629600029
dc.identifier10.1021/bm100570r
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/55487
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/55487
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/55487
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1265321
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionA strategy was developed to produce thin, biopolymer-based polyelectrolyte multilayer films, based on hyaluronic acid and chitosan, that are able to effectively bind B lymphocytes. These films explore CD44-hyaluronate interactions and provide a method to make surface-bound B cell arrays without the need for nonselective covalent chemistry. The rational design of these films using solution deposition variables, such as ionic strength and pH, allows one to maximize and fine tune this binding efficiency ex vivo. This work suggests two important conditions for successfully attaching B cells to hyaluronate-containing polyelectrolyte multilayer films: (1) hyaluronic acid is required for the proposed CD44-mediated binding mechanism, and (2) hyaluronic acid deposition conditions that favor loops and tails, such as low pH and with added salt, result in more available CD44 binding ligands and higher cell binding efficiency. Chitosan-terminated films prepared without NaCl in the deposition solutions and hyaluronic acid-terminated films prepared with salt, both under pH 3.0 assembly conditions, presented a similar high lymphocyte binding efficiency. In the former case, however, the binding strength was weaker due to a significant electrostatic contribution to the binding. Bioactive polyelectrolyte multilayers for selective binding of lymphocytes hold great promise in fields ranging from cell-based biosensors to immune system engineering.
dc.description11
dc.description9
dc.description2407
dc.description2414
dc.descriptionNational Science Foundation [DMR-0819762]
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionNational Science Foundation [DMR-0819762]
dc.descriptionCAPES [1101-08-0]
dc.languageen
dc.publisherAmer Chemical Soc
dc.publisherWashington
dc.publisherEUA
dc.relationBiomacromolecules
dc.relationBiomacromolecules
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectChitosan-chondroitin Sulfate
dc.subjectLiving Cells
dc.subjectIn-vitro
dc.subjectSurface Functionalization
dc.subjectWeak Polyelectrolytes
dc.subjectFilms
dc.subjectComplexes
dc.subjectGrowth
dc.subjectBiodegradability
dc.subjectDegradability
dc.titleBioactive Polyelectrolyte Multilayers: Hyaluronic Acid Mediated B Lymphocyte Adhesion
dc.typeArtículos de revistas


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