dc.creatorGirasol, A
dc.creatorAlbuquerque, GG
dc.creatorMansour, E
dc.creatorAraujo, EP
dc.creatorDegasperi, G
dc.creatorDenis, RG
dc.creatorCarvalheira, JB
dc.creatorSaad, MJ
dc.creatorVelloso, LA
dc.date2009
dc.date37926
dc.date2014-11-14T00:39:12Z
dc.date2015-11-26T16:03:34Z
dc.date2014-11-14T00:39:12Z
dc.date2015-11-26T16:03:34Z
dc.date.accessioned2018-03-28T22:52:47Z
dc.date.available2018-03-28T22:52:47Z
dc.identifierPlos One. Public Library Science, v. 4, n. 11, 2009.
dc.identifier1932-6203
dc.identifierWOS:000271414500009
dc.identifier10.1371/journal.pone.0007707
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/67733
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/67733
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/67733
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1265296
dc.descriptionBackground: Several effects of leptin in the immune system rely on its capacity to modulate cytokine expression and apoptosis in the thymus. Surprisingly, some of these effects are dependent on signal transduction through the IRS1/PI3-kinase, but not on the activation of JAK2. Since all the well known effects of leptin in different cell types and tissues seem to be dependent on JAK2 activation, we hypothesized that, at least for the control of thymic function, another, unknown kinase could mediate the transduction of the leptin signal from the ObR towards the IRS1/PI3-kinase signaling cascade. Methodology/Principal Findings: Here, by employing immunoblot, real-time PCR and flow citometry we show that the tyrosine kinase, Fyn, is constitutively associated with the ObR in thymic cells. Following a leptin stimulus, Fyn undergoes an activating tyrosine phosphorylation and a transient association with IRS1. All these effects are independent of JAK2 activation and, upon Fyn inhibition, the signal transduction towards IRS1/PI3-kinase is abolished. In addition, the inhibition of Fyn significantly modifies the effects of leptin on thymic cytokine expression. Conclusion /Significance: Therefore, in the thymus, Fyn acts as a tyrosine kinase that transduces the leptin signal independently of JAK2 activation, and mediates some of the immunomodulatory effects of leptin in this tissue.
dc.description4
dc.description11
dc.languageen
dc.publisherPublic Library Science
dc.publisherSan Francisco
dc.publisherEUA
dc.relationPlos One
dc.relationPLoS One
dc.rightsaberto
dc.sourceWeb of Science
dc.subjectPeripheral T-cells
dc.subjectImmune-response
dc.subjectReceptor
dc.subjectActivation
dc.subjectHypothalamus
dc.subjectImmunology
dc.subjectResistance
dc.subjectNutrition
dc.subjectPathways
dc.subjectP59(fyn)
dc.titleFyn Mediates Leptin Actions in the Thymus of Rodents
dc.typeArtículos de revistas


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