Histoenzymological and ultrastructural changes in lateral muscle fibers of Oreochromis niloticus (Teleostei : Cichlidae) after local injection of veratrine

dc.creatorFreitas, EMS
dc.creatorSilva, MD
dc.creatorda Cruz-Hofling, MA
dc.date2001
dc.dateDEC
dc.date2014-11-13T23:41:36Z
dc.date2015-11-26T16:03:18Z
dc.date2014-11-13T23:41:36Z
dc.date2015-11-26T16:03:18Z
dc.date.accessioned2018-03-28T22:52:33Z
dc.date.available2018-03-28T22:52:33Z
dc.identifierHistochemistry And Cell Biology. Springer, v. 116, n. 6, n. 525, n. 534, 2001.
dc.identifier0948-6143
dc.identifier1432-119X
dc.identifierWOS:000173198100007
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/69853
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/69853
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/69853
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1265238
dc.descriptionThe effects of veratrine have been investigated in mammalian, amphibian, and crustacean muscle, but not in fish. In this work, the action of veratrine was studied in the lateral muscle of the freshwater teleost Oreochromis niloticus after intramuscular injection. Histoenzymological typing and electron microscopy of muscle fibers before and 15, 30, and 60 min after veratrine injection (10 ng/kg fish) were used to indirectly assess the morphological changes and the oxidative and m-ATPase activities. In some cases, muscles were pretreated with tetrodotoxin to determine whether the ultrastructural changes were the result of Na+ channel activation by veratrine. Veratrine altered the metabolism of fibers mainly after 30 min. Oxidative fibers showed decreased NADH-TR activity, whereas that of glycolytic and oxidative-glycolytic type fibers increased. There was no change in the m-ATPase activity of the three fiber types, except at 60 min postveratrine, when a novel fiber type, which showed no reversal after acidic and alkaline preincubations, appeared. Ultrastructural damage involved sarcomeres, myofibrils, and mitochondria, but the T-tubules remained intact. Pretreatment with tetrodotoxin (1 ng/ml) prevented the ultrastructural changes caused by veratrine, These results show that in fish skeletal muscle veratrine produces some effects that are not seen in mammalian muscle.
dc.description116
dc.description6
dc.description525
dc.description534
dc.languageen
dc.publisherSpringer
dc.publisherNew York
dc.publisherEUA
dc.relationHistochemistry And Cell Biology
dc.relationHistochem. Cell Biol.
dc.rightsfechado
dc.rightshttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.sourceWeb of Science
dc.subjectfish
dc.subjecthistoenzymology
dc.subjectmuscle plasticity
dc.subjectultrastructural changes
dc.subjectveratrine
dc.subjectSkeletal-muscle
dc.subjectTemperature-acclimation
dc.subjectSarcoplasmic-reticulum
dc.subjectAnguilla-anguilla
dc.subjectActomyosin Atpase
dc.subjectMyotomal Muscle
dc.subjectWhite Muscle
dc.subjectCarp
dc.subjectChannels
dc.subjectVenom
dc.titleHistoenzymological and ultrastructural changes in lateral muscle fibers of Oreochromis niloticus (Teleostei : Cichlidae) after local injection of veratrine
dc.typeArtículos de revistas


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