Extrusion of fluorescein diacetate by multidrug-resistant Candida albicans

dc.creatorYang, HC
dc.creatorMikami, Y
dc.creatorImai, T
dc.creatorTaguchi, H
dc.creatorNishimura, K
dc.creatorMiyaji, M
dc.creatorBranchini, MLM
dc.date2001
dc.date2014-11-13T23:12:12Z
dc.date2015-11-26T16:03:07Z
dc.date2014-11-13T23:12:12Z
dc.date2015-11-26T16:03:07Z
dc.date.accessioned2018-03-28T22:52:26Z
dc.date.available2018-03-28T22:52:26Z
dc.identifierMycoses. Blackwell Wissenschafts-verlag Gmbh, v. 44, n. 41921, n. 368, n. 374, 2001.
dc.identifier0933-7407
dc.identifierWOS:000172652800004
dc.identifier10.1046/j.1439-0507.2001.00666.x
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/66620
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/66620
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/66620
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1265204
dc.descriptionOver-expression of multidrug efflux transporters causes Candida albicans cells to be resistant to azole antifungal agents. There are several kinds of indicator for multidrug resistance (MDR.) phenotype of higher eukaryotic cells. Calcein AM is a prefluorochrome that is known as a substrate for multidrug efflux transporters of mammalian cells. We investigated whether calcein AXI was also extruded by the ATP-dependent multidrug transporter (cdr1p) of C. albicans There was no significant difference in the accumulation of calcein AM between MDR cells and drug-susceptible cells of C. albicans even with sodium azide, suggesting that calcein Am may not be associated with the CDR1-gene-related multidrug efflux system of C. albicans. However, a structurally related prefluorochrome derivative, fluorescein diacetate (FDA), was shown to be extruded by the CDR1 mRNA-overexpressing yeast cells. In comparison with drug-susceptible cells, the resistant cells emitted very weak fluorescence when stained with FDA. Furthermore sodium azide increased the fluorescence of the resistant cells more than 20 times, whereas the fluorescence in the drug-susceptible cells with FDA and sodium azide was three to four times stronger. These results suggested that FDA might be extruded by the CDR1-related multidrug efflux transporter of C. albicans.
dc.description44
dc.description41921
dc.description368
dc.description374
dc.languageen
dc.publisherBlackwell Wissenschafts-verlag Gmbh
dc.publisherBerlin
dc.publisherAlemanha
dc.relationMycoses
dc.relationMycoses
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectCandida albicans
dc.subjectmultidrug resistance
dc.subjectCDR1
dc.subjectcalcein AM
dc.subjectfluorescein diacetate
dc.subjectAzole Antifungal Agents
dc.subjectDrug-resistance
dc.subjectP-glycoprotein
dc.subjectEfflux
dc.subjectFluconazole
dc.subjectMechanisms
dc.subjectAccumulation
dc.titleExtrusion of fluorescein diacetate by multidrug-resistant Candida albicans
dc.typeArtículos de revistas


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