Porphyromonas gingivalis, Porphyromonas endodontalis, Prevotella intermedia and Prevotella nigrescens in endodontic lesions detected by culture and by PCR

dc.creatorGomes, BPFA
dc.creatorJacinto, RC
dc.creatorPinheiro, ET
dc.creatorSousa, ELR
dc.creatorZaia, AA
dc.creatorFerraz, CCR
dc.creatorSouza, FJ
dc.date2005
dc.dateAUG
dc.date2014-11-13T22:24:05Z
dc.date2015-11-26T16:02:43Z
dc.date2014-11-13T22:24:05Z
dc.date2015-11-26T16:02:43Z
dc.date.accessioned2018-03-28T22:52:06Z
dc.date.available2018-03-28T22:52:06Z
dc.identifierOral Microbiology And Immunology. Blackwell Publishing, v. 20, n. 4, n. 211, n. 215, 2005.
dc.identifier0902-0055
dc.identifierWOS:000229642900004
dc.identifier10.1111/j.1399-302X.2005.00214.x
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/81447
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/81447
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/81447
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1265127
dc.descriptionThe aim of this study was to investigate the presence of four black-pigmented bacteria, Porphyromonas gingivalis, Porphyromonas endodontalis, Prevotella intermedia and Prevotella nigrescens, in endodontic infections by culture and polymerase chain reaction (PCR) analyses. Microbial samples were obtained from 50 teeth with untreated necrotic pulps (primary infection) and from 50 teeth with failing endodontic treatment (secondary infection). Microbiological strict anaerobic techniques were used for serial dilution, plating, incubation, and identification. For PCR detection, the samples were analyzed using species-specific primers of 16S rDNA and the downstream intergenic spacer region. Culture and PCR detected the test species in 13/100 and 50/100 of the study teeth, respectively. The organisms were cultured from 11/50 (22%) of primarily infected root canal samples and from 2/50 (4%) of secondary root canal samples. PCR detection identified the target species in 32/50 (64%) and 18/50 (36%) of primary and secondary infections, respectively. P. gingivalis was rarely isolated by culture methods (1%), but was the most frequently identified test species by PCR (38%). Similarly, P. endodontalis was not recovered by culture from any tooth studied, but was detected by PCR in 25% of the sampled teeth. PCR-based identification also showed higher detection rates of P. intermedia (33%) and P. nigrescens (22%) than culture (13%). In conclusion, P. gingivalis, P. endodontalis, P. intermedia, and P. nigrescens were identified more frequently in teeth with necrotic pulp than in teeth with failing endodontic treatment. Also, a higher frequency of black-pigmented species was detected by PCR than by culture.
dc.description20
dc.description4
dc.description211
dc.description215
dc.languageen
dc.publisherBlackwell Publishing
dc.publisherOxford
dc.publisherInglaterra
dc.relationOral Microbiology And Immunology
dc.relationOral Microbiol. Immunol.
dc.rightsfechado
dc.sourceWeb of Science
dc.subjectblack-pigmented anaerobes
dc.subjectendodontic pathosis
dc.subjectPorphyromonas
dc.subjectPrevotella
dc.subjectPolymerase-chain-reaction
dc.subjectBlack-pigmented Bacteria
dc.subjectInfected Root Canals
dc.subjectAcute Periradicular Abscesses
dc.subjectAnaerobic-bacteria
dc.subjectSymptoms
dc.subjectIdentification
dc.subjectPeriodontitis
dc.subjectMicroorganisms
dc.subjectCombinations
dc.titlePorphyromonas gingivalis, Porphyromonas endodontalis, Prevotella intermedia and Prevotella nigrescens in endodontic lesions detected by culture and by PCR
dc.typeArtículos de revistas


Este ítem pertenece a la siguiente institución