Immunochemical detection of Lonomia obliqua caterpillar venom in rats

dc.creatorDa Silva, GH
dc.creatorPanunto, PC
dc.creatorHyslop, S
dc.creatorDa Cruz-Hofling, MA
dc.date2004
dc.dateDEC
dc.date2014-11-13T20:54:38Z
dc.date2015-11-26T16:02:36Z
dc.date2014-11-13T20:54:38Z
dc.date2015-11-26T16:02:36Z
dc.date.accessioned2018-03-28T22:52:01Z
dc.date.available2018-03-28T22:52:01Z
dc.identifierMicroscopy Research And Technique. Wiley-liss, v. 65, n. 6, n. 276, n. 281, 2004.
dc.identifier1059-910X
dc.identifierWOS:000226907000003
dc.identifier10.1002/jemt.20114
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/69932
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/69932
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/69932
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1265100
dc.descriptionSevere cases of human envenoming by caterpillars of the saturniid moth Lonomia obliqua in Brazil can result in renal damage, leading to renal failure, and intracerebral hemorrhaging. In this work, we used immunohistochemical staining with rabbit antiserum raised against L. obliqua venom to examine venom distribution in selected tissues of the brain (cerebellum and hippocampus), kidneys, and liver of male Wistar rats injected with a single dose of venom (200 mug/kg, i.v.) and sacrificed 6, 18, 24, and 72 hours later. The immunolabeling of GFAP was also examined to assess the venom effects on perivascular astrocytic end-feet in the microvasculature of the hippocampus and cerebellum. Venom was detected in the kidneys (6 and 18 hours) and in the liver (6 hours) but not in the brain at any of the time intervals examined. In contrast, immunolabeling for GFAP revealed astrogliosis in the cerebellum and enhanced expression of this protein in the glial processes of the cerebellum and hippocampus, with a maximum response from 24 hours onwards. The high immunoreactivity seen in the kidneys agreed with the renal damage and dysfunction reported for some patients. The lack of venom detection in the brain, despite the altered expression of GFAP in astrocytes, suggested either that the venom does not enter this organ or that its entrance is transient and fast. Alternatively, the circulating venom may induce the release of mediators that could serve as second messengers to provoke the late astrocytic reactivity and astrogliosis. It is possible that both of these mechanisms may contribute to the effects observed. (C) 2005 Wiley-Liss, Inc.
dc.description65
dc.description6
dc.description276
dc.description281
dc.languageen
dc.publisherWiley-liss
dc.publisherHoboken
dc.publisherEUA
dc.relationMicroscopy Research And Technique
dc.relationMicrosc. Res. Tech.
dc.rightsfechado
dc.rightshttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.sourceWeb of Science
dc.subjectbrain
dc.subjectglial fibrillary acidic protein (GFAP)
dc.subjectkidney
dc.subjectliver
dc.subjectLonomia obliqua
dc.subjectvenom distribution
dc.subjectSerrulatus Scorpion-venom
dc.subjectActivator Serine-protease
dc.subjectHemorrhagic Syndrome
dc.subjectBlood-coagulation
dc.subjectBody Distribution
dc.subjectProthrombin
dc.subjectLopap
dc.subjectPharmacokinetics
dc.subjectEnvenomation
dc.subjectF(ab')(2)
dc.titleImmunochemical detection of Lonomia obliqua caterpillar venom in rats
dc.typeArtículos de revistas


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