DNA content, chromatin texture and nuclear morphology in benzo[a]pyrene-transformed human breast epithelial cells after microcell-mediated transfer of chromosomes 11 and 17

dc.creatorMello, MLS
dc.creatorVidal, BD
dc.creatorLareef, MH
dc.creatorHu, YF
dc.creatorYang, XQ
dc.creatorRusso, J
dc.date2003
dc.dateAPR
dc.date2014-11-13T19:36:29Z
dc.date2015-11-26T16:02:09Z
dc.date2014-11-13T19:36:29Z
dc.date2015-11-26T16:02:09Z
dc.date.accessioned2018-03-28T22:51:36Z
dc.date.available2018-03-28T22:51:36Z
dc.identifierCytometry Part A. Wiley-liss, v. 52A, n. 2, n. 70, n. 76, 2003.
dc.identifier0196-4763
dc.identifierWOS:000182452800002
dc.identifier10.1002/cyto.a.10029
dc.identifierhttp://www.repositorio.unicamp.br/jspui/handle/REPOSIP/62396
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/62396
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/62396
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1265000
dc.descriptionBackground: A relation between the changes in DNA content and chromatin supra-organization and the expression of gradual steps of tumorigenesis has been assessed by image analysis in human breast epithelial cells (MCF-10F) treated with benzo[a]pyrene (BP) (cell lines BP1, BP1-E, BP1-Tras, and-others). Methods: Because abnormal chromosomes 11 and 17 have been associated with neoplastic progression in BP-transformed MCF-10F cells, image analysis of Feulgen-stained tumorigenic BP1-E cells with the microcell-mediated chromosome transfer of normal chromosomes 11 and 17 was carried out. Results: A tendency of DNA amount distribution and nuclear size restoration to values typical of non-transformed MCF-10F cells Was demonstrated, especially after the transfer of chromosome 17. No reversion in chromatin texture was found after the transfer of chromosome 11 or 17. Conclusions: Although the presence of a normal chromosome 17 should be considered among the necessary steps for tumorigenic human breast epithelial cells to recover their normality, a more complex genome balance is required for the entire nuclear chromatin of these cells to recover its totally normal supra-organization and expression. (C) 2003 Wiley-Liss, Inc.
dc.description52A
dc.description2
dc.description70
dc.description76
dc.languageen
dc.publisherWiley-liss
dc.publisherNew York
dc.publisherEUA
dc.relationCytometry Part A
dc.relationCytom. Part A
dc.rightsfechado
dc.rightshttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.sourceWeb of Science
dc.subjectDNA content
dc.subjectchromatin texture
dc.subjecttumorigenesis
dc.subjecthuman breast epithelial cells
dc.subjectchromosomes 11 and 17
dc.subjectimage analysis
dc.subjectHa-ras Oncogene
dc.subjectMicrosatellite Instability
dc.subjectChemical-carcinogens
dc.subjectImage-analysis
dc.subjectIn-vitro
dc.subjectRepair Deficiency
dc.subjectCancer
dc.subjectTransformation
dc.subjectSupraorganization
dc.subjectMutation
dc.titleDNA content, chromatin texture and nuclear morphology in benzo[a]pyrene-transformed human breast epithelial cells after microcell-mediated transfer of chromosomes 11 and 17
dc.typeArtículos de revistas


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