dc.creator | Almeida, AC | |
dc.creator | Rehder, J | |
dc.creator | Severino, SD | |
dc.creator | Martins, J | |
dc.creator | Newburger, PE | |
dc.creator | Condino-Neto, A | |
dc.date | 2005 | |
dc.date | SEP | |
dc.date | 2014-11-13T17:33:27Z | |
dc.date | 2015-11-26T16:01:38Z | |
dc.date | 2014-11-13T17:33:27Z | |
dc.date | 2015-11-26T16:01:38Z | |
dc.date.accessioned | 2018-03-28T22:51:11Z | |
dc.date.available | 2018-03-28T22:51:11Z | |
dc.identifier | Journal Of Interferon And Cytokine Research. Mary Ann Liebert Inc, v. 25, n. 9, n. 540, n. 546, 2005. | |
dc.identifier | 1079-9907 | |
dc.identifier | WOS:000232229200003 | |
dc.identifier | 10.1089/jir.2005.25.540 | |
dc.identifier | http://www.repositorio.unicamp.br/jspui/handle/REPOSIP/75055 | |
dc.identifier | http://www.repositorio.unicamp.br/handle/REPOSIP/75055 | |
dc.identifier | http://repositorio.unicamp.br/jspui/handle/REPOSIP/75055 | |
dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/1264895 | |
dc.description | The aim of this work was to analyze the effect of Interferon-gamma ( IFN-gamma) and tumor necrosis factor-alpha ( TNF-alpha) on NADPH oxidase activity and gp91- phox gene expression in human colostrum macrophages ( CM), peripheral blood monocytes ( PBM), and myelomonocytic THP- 1 cells. We also investigated the effect of IFN-gamma on the release of TNF-alpha by these cells. Our results show that under basal culture conditions, CM release more superoxide than PBM and THP- 1 cells ( p < 0.05). The addition of IFN-gamma, alone or in combination with TNF-alpha, increased spontaneous superoxide release by PBM and THP- 1 cells ( p < 0.05) and increased phorbol myristate acetate ( PMA)- stimulated superoxide release by CM, PBM, and THP- 1 cells ( p < 0.05). The NADPH oxidase activity of THP- 1 cells consistently remained lower than that of CM or PBM, despite a dramatic response to IFN-gamma and TNF-alpha. Under basal conditions, gp91- phox gene expression was significantly higher in CM and PBM compared with THP- 1 cells ( p < 0.05). The addition of IFN-gamma alone or in combination with TNF-alpha caused a dramatic increase in gp91- phox gene expression in THP- 1 cells ( p < 0.05) but not in CM or PBM. Under basal conditions or in the presence of IFN-gamma, CM released more TNF-alpha than PBM or THP- 1 cells ( p < 0.05). In addition, PBM released more TNF-alpha than THP- 1 cells ( p < 0.05). IFN-gamma did not significantly augment the release of TNF-alpha by these cells ( p > 0.05). Thus, IFN-gamma and TNF-alpha induced equivalent gp91- phox gene expression in THP- 1 cells compared with CM or PBM but did not bring about equivalent NADPH oxidase activity. TNF-alpha release was higher in more mature cells. This partial divergence of gp91-phox gene expression, NADPH oxidase activity, and TNF-alpha release is probably a consequence of different events of myeloid cell biology and relates at least in part to cell differentiation state. | |
dc.description | 25 | |
dc.description | 9 | |
dc.description | 540 | |
dc.description | 546 | |
dc.language | en | |
dc.publisher | Mary Ann Liebert Inc | |
dc.publisher | New Rochelle | |
dc.publisher | EUA | |
dc.relation | Journal Of Interferon And Cytokine Research | |
dc.relation | J. Interferon Cytokine Res. | |
dc.rights | aberto | |
dc.source | Web of Science | |
dc.subject | Human-milk Macrophages | |
dc.subject | Chain Gene-expression | |
dc.subject | Necrosis-factor-alpha | |
dc.subject | Cytochrome-b Heavy | |
dc.subject | Nf-kappa-b | |
dc.subject | Superoxide Generation | |
dc.subject | Interferon-gamma | |
dc.subject | Cyto-toxicity | |
dc.subject | Lipopolysaccharide | |
dc.subject | Differentiation | |
dc.title | The effect of IFN-gamma and TNF-alpha on the NADPH oxidase system of human colostrum macrophages, blood monocytes, and THP-1 cells | |
dc.type | Artículos de revistas | |