Artículos de revistas
In Vivo Apoptosis Induction And Reduction Of Infectivity By An Autographa Californica Multiple Nucleopolyhedrovirus P35- Recombinant In Hemocytes From The Velvet Bean Caterpillar Anticarsia Gemmatalis (hübner) (lepidoptera: Noctuidae)
Registro en:
Research In Microbiology. , v. 156, n. 10, p. 1014 - 1025, 2005.
9232508
10.1016/j.resmic.2005.06.001
2-s2.0-28444497457
Autor
Da Silveira E.B.
Cordeiro B.A.
Ribeiro B.M.
Bao S.N.
Institución
Resumen
Baculoviruses have long been shown to regulate apoptosis in cultured insect cells. Recently, this phenomenon was also reported to occur in vivo, reinforcing the importance of apoptosis in insect immunity against viruses. The vP35del virus, an Autographa californica multiple nucleopolyhedrovirus (AcMNPV) recombinant, was previously shown to induce apoptosis in Anticarsia gemmatalis cultured cells. In order to verify the AcMNPV interaction with hemocytes, apoptosis induction in vivo and its effects upon infectivity, we studied the course of intrahemocoelic infection of recombinant viruses (vHSGFP and vHSGFP/P35del) in A. gemmatalis larvae. Insect development and mortality were monitored and infection progress was followed by light, fluorescence and electron microscopy. For all doses tested, vHSGFP/P35del caused lower mortality than vHSGFP. Mortality of 95% occurred with a dose of 4×106 PFUs of vHSGFP, which was reduced to 60% for vHSGFP/P35del. GFP expression was first observed at 3 h p.i. for the two viruses, increasing for vHSGFP (40% at 120 h p.i.) and decreasing for vHSGFP/P35del (0% at 120 h p.i.). The virus vHSGFP/P35del induced apoptosis in hemocytes, with some budded virus being produced, and fragmented cells were observed between 24 and 72 h p.i. The recombinant vHSGFP induced typical wild-type cytopathic effects, with low production of occluded viruses until 120 h p.i. Plasmatocytes and granular hemocytes type 1 were the hemocytes most susceptible to both viruses. For these experimental conditions, we concluded that A. gemmatalis is a semi-permissive host to AcMNPV; moreover, apoptosis reduces AcMNPV infectivity and the p35 gene is essential for blocking apoptosis in this system. © 2005 Elsevier SAS. All rights reserved. 156 10 1014 1025 Barrett, J.W., Brownwright, A.J., Primavera, M.J., Retnakaran, A., Palli, S.R., Concomitant primary infection of the midgut epithelial cells and the hemocytes of Trichoplusia ni by Autographa californica nucleopolyhedrovirus (1998) Tissue Cell, 30, pp. 602-616 Black, B.C., Brennan, L.A., Dierks, P.M., Gard, I.E., Commercialization of baculoviral insecticides (1997) The Baculoviruses, pp. 341-387. , L.K. Miller Plenum Press New York Blissard, G.W., Black, B., Crook, N., Keddie, B.A., Possee, R., Rohrmann, G., Theilmann, D., Volkman, L., (2000) Virus Taxonomy: Seventh Report of the International Comittee on Taxonomy of Viruses, pp. 195-202. , M.H.V. Van Regenmortel C.M. Fauquet D.H.L. Bishop E.B. Carstens M.K. Estes S.M. Lemon J. Maniloff M.A. Mayo D.J. McGeoch C.R. Pringle R.B. Wickner Academic Press San Diego Castro, M.E.B., Ribeiro, B.M., Production of viral progeny in insect cells undergoing apoptosis induced by a mutant Anticarsia gemmatalis nucleopolyhedrovirus (2001) Microbiol. Res., 156, pp. 369-376 Chejanovsky, N., Gershburg, E., The wild-type Autographa californica nuclear polyhedrosis virus induces apoptosis of Spodoptera littoralis cells (1995) Virology, 209, pp. 519-525 Clarke, T.E., Clem, R.J., Lack of involvement of haemocytes in the establishment and spread of infection in Spodoptera frugiperda larvae infected with the baculovirus Autographa californica M nucleopolyhedrovirus by intrahaemocolic injection (2002) J. Gen. Virol., 83, pp. 1565-1572 Clarke, T.E., Clem, R.J., Insect defenses against virus infection: The role of apoptosis (2003) Int. Rev. Immunol., 22, pp. 401-424 Clarke, T.E., Clem, R.J., In vivo induction of apoptosis correlating with reduced infectivity during baculovirus infection (2003) J. Virol., 77, pp. 2227-2232 Clem, R.J., Baculoviruses and apoptosis: The good, the bad and the ugly (2001) Cell Death Differ., 8, pp. 137-143 Clem, R.J., Fechheimer, M., Miller, L.K., Prevention of apoptosis by a baculovirus gene during infection of insect cells (1991) Science, 254, pp. 1388-1390 Clem, R.J., Miller, L.K., Apoptosis reduces both the in vitro replication and the in vivo infectivity of a baculovirus (1993) J. Virol., 67, pp. 3730-3738 Clem, R.J., Robson, M., Miller, L.K., Influence of infection route on the infectivity of baculovirus mutants lacking the apoptosis-inhibiting gene p35 and the adjacent gene p94 (1994) J. Virol., 68, pp. 6759-6762 Dai, X., Shi, X., Pang, Y., Su, D., Prevention of baculovirus-induced apoptosis of BTI-Tn-5B1-4 (Hi5) cells by the p35 gene of Trichoplusia ni multicapsid nucleopolyhedrovirus (1999) J. Gen. Virol., 80, pp. 1841-1845 Du, Q., Lehavi, D., Faktor, O., Qi, Y., Chejanovsky, N., Isolation of an apoptosis suppressor gene of the Spodoptera littoralis nucleopolyhedrovirus (1999) J. Virol., 73, pp. 1278-1285 Granados, R.R., Guoxun, L., Derksen, A.C.G., McKenna, K.A., A new insect cell line from Trichoplusia ni (BTI-Tn-5B1-4) susceptible to Trichoplusia ni single enveloped nuclear polyhedrosis virus (1994) J. Invertebr. Pathol., 64, pp. 260-266 Granados, R.R., Lawler, K.A., In vivo pathway of Autographa californica baculovirus invasion and infection (1981) Virology, 108, pp. 297-308 Greene, G.L., Leppla, N.C., Dickerson, W.A., Velvetbean caterpillar: A rearing procedure and artificial medium (1976) J. Econ. Entomol., 69, pp. 487-488 Jarvis, D.L., Baculovirus expression vectors (1997) The Baculoviruses, pp. 237-266. , L.K. Miller Plenum Press New York Kamita, S.G., Majima, K., Maeda, S., Identification and characterization of the p35 gene of the Bombyx mori nuclear polyhedrosis virus that prevents virus-induced apoptosis (1993) J. Virol., 67, pp. 455-463 Kislev, N., Harpaz, I., Zelcer, A., Electron-microscopic studies on haemocytes of the Egyptian Cottonworm, Spodoptera litorallis (Boisduval) infected with a nuclear-polyhedrosis virus, as compared to noninfected hemocytes. II. Virus-infected hemocytes (1969) J. Invertebr. Pathol., 14, pp. 245-257 Koyama, A.H., Fukumori, T., Fujita, M., Irie, H., Adachi, A., Physiological significance of apoptosis in animal virus infection (2000) Microbes Infect., 2, pp. 1111-1117 Lavine, M.D., Strand, M.R., Insect hemocytes and their role in immunity (2002) Insect Biochem. Mol. Biol., 32, pp. 1295-1309 Mead, G.P., Ratcliffe, N.A., Renwrantz, L.R., The separation of insect hemocyte types on Percoll gradients: Methodology and problems (1986) J. Insect Physiol., 32, pp. 167-177 Moscardi, F., Assessment of the application of baculoviruses for control of lepidoptera (1999) Annu. Rev. Entomol., 44, pp. 257-289 O'Reilly, D.R., Miller, L.K., A baculovirus blocks insect molting by producing ecdysteroid UDP-glucosyl transferase (1989) Science, 245, pp. 1110-1112 O'Reilly Miller, L.K.D.R., Luckow, V.A., (1992) Baculovirus Expression Vectors: A Laboratory Manual, , Freeman New York Palli, S.R., Caputo, G.F., Sohi, S.S., Brownright, A.J., Ladd, T.R., Cook, B.J., Primavera, M., Retnakaran, A., CfMNPV blocks AcMNPV-induced apoptosis in a continuous midgut cell line (1996) Virology, 222, pp. 201-213 Pang, Y., Yu, J.X., Wang, L.H., Hu, X.H., Bao, W.D., Li, G., Chen, C., Yang, H.M., Sequence analysis of the Spopdoptera litura multicapsid nucleopolyhedrovirus genome (2001) Virology, 287, pp. 391-404 Sahdev, S., Taneja, T.K., Mohan, M., Sah, N.K., Khar, A.K., Hasnain, S.E., Athar, M., Baculoviral p35 inhibits oxidant-induced activation of mitochondrial apoptotic pathway (2003) Biochem. Biophys. Res. Comm., 307, pp. 483-490 Sieburth, P.J., Maruniak, J.E., Growth characteristics of a continuous line from the velvetbean caterpillar, Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae) (1988) In Vitro Cell Dev. Biol., 24, pp. 195-198 Sieburth, P.J., Maruniak, J.E., Susceptibility of an established cell line of Anticarsia gemmatalis (Lepidoptera: Noctuidae) to three nuclear polyhedrosis viruses (1988) J. Invertebr. Pathol., 52, pp. 453-458 Silveira, E.B., Cordeiro, B.A., Ribeiro, B.M., Báo, S.N., Morphological characterization of Anticarsia gemmatalis M nucleopolyhedrovirus infection in haemocytes from its natural larval host, the velvet bean caterpillar Anticarsia gemmatalis (Hübner) (Lepidoptera: Noctuidae) (2004) Tissue Cell, 36, pp. 171-180 Silveira, E.B., Ribeiro, B.M., Báo, S.N., Morphological studies of apoptosis in insect cells infected with vApAg, an Anticarsia gemmatalis nucleopolyhedrovirus mutant (1999) J. Submicrosc. Cytol. Pathol., 31, pp. 543-554 Silveira, E.B., Ribeiro, B.M., Báo, S.N., Characterization of larval haemocytes from the velvetbean caterpillar Anticarsia gemmatalis (Hübner) (Lepidoptera: Noctuidae) (2003) J. Submicrosc. Cytol. Pathol., 35, pp. 129-139 Teodoro, J.G., Branton, P.E., Regulation of apoptosis by viral gene products (1997) J. Virol., 71, pp. 1739-1746 Trudeau, D., Washburn, J.O., Volkman, L.E., Central role of haemocytes in Autographa californica M nucleopolyhedrosis pathogenesis in Heliothis virescens and Helicoverpa zea (2001) J. Virol., 75, pp. 996-1003 Washburn, J.O., Haas-Stapleton, E.J., Tan, F.F., Beckage, N.E., Volkman, L.E., Co-infection of Manduca sexta larvae with polydnavirus from Cotesia congregata increases susceptibility to fatal infection by Autographa californica M nucleopolyhedrovirus (2000) J. Insect Physiol., 46, pp. 179-190 Washburn, J.O., Kirkpatrick, B.A., Volkman, L.E., Insect protection against viruses (1996) Nature, 383, p. 767 Washburn, J.O., Kirkpatrick, B.A., Volkman, L.E., Comparative pathogenesis of Autographa californica M nuclear polyhedrosis virus in larvae of Trichoplusia ni and Heliothis virescens (1995) Virology, 209, pp. 561-568 Zhang, P., Yang, K., Daí, X., Pang, Y., Su, D., Infection of wild-type Autographa californica multicapsid nucleopolyhedrovirus induces in vivo apoptosis of Spodoptera litura larvae (2002) J. Gen. Virol., 83, pp. 3003-3011 Zhang, P., Yang, B., Dai, X.J., Pang, Y., Zhong, J., Su, D.M., Apoptosis of Spodoptera litura cells induced by AcMNPV ie-1 gene (2002) Acta Biochim. Biophys. Sin., 34, pp. 707-711