Pharmacological And Structural Characterization Of A Novel Phospholipase A2 From Micrurus Dumerilii Carinicauda Venom

dc.creatorDal Belo C.A.
dc.creatorLeite G.B.
dc.creatorToyama M.H.
dc.creatorMarangoni S.
dc.creatorCorrado A.P.
dc.creatorFontana M.D.
dc.creatorSouthan A.
dc.creatorRowan E.G.
dc.creatorHyslop S.
dc.creatorRodrigues-Simioni L.
dc.date2005
dc.date2015-06-26T14:06:35Z
dc.date2015-11-26T15:40:18Z
dc.date2015-06-26T14:06:35Z
dc.date2015-11-26T15:40:18Z
dc.date.accessioned2018-03-28T22:48:48Z
dc.date.available2018-03-28T22:48:48Z
dc.identifier
dc.identifierToxicon. , v. 46, n. 7, p. 736 - 750, 2005.
dc.identifier410101
dc.identifier10.1016/j.toxicon.2005.07.016
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-27544437523&partnerID=40&md5=961b3571f0f2d4f6252de54c1e800eaa
dc.identifierhttp://www.repositorio.unicamp.br/handle/REPOSIP/93151
dc.identifierhttp://repositorio.unicamp.br/jspui/handle/REPOSIP/93151
dc.identifier2-s2.0-27544437523
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/1264346
dc.descriptionWe have isolated a new phospholipase A2 (MiDCA1) from the venom of the coral snake Micrurus dumerilii carinicauda. This toxin, which had a molecular mass of 15,552 Da, shared high sequence homology with the PLA 2 toxins MICNI A and B from Micrurus nigrocinctus venom (77.7% and 73.1%, respectively). In chick biventer cervicis preparations, MiDCA1 produced concentration- and time-dependent neuromuscular blockade that reached 100% after 120 min (2.4 μM, n=6); contractures to exogenously applied carbachol (8 μM) and KCl (13 mM) were still seen after complete blockade. In mouse phrenic-nerve diaphragm preparations, MiDCA1 (2.4 μM; n=6) caused triphasic changes followed by partial neuromuscular blockade. Intracellular recordings of end-plate potentials (EPPs) and miniature end-plate potentials (MEPPs) from mouse diaphragm preparations showed that MiDCA1 increased the quantal content by 386±12% after 10 min (n=14; p<0.05) and caused a triphasic change in the frequency of MEPPs. MiDCA1 also decreased the resting membrane potential, an effect that was prevented by tetrodotoxin and/or low extracellular calcium, but not by d-tubocurarine. The toxin increased the amplitude of mouse sciatic-nerve compound action potentials by 30±9% (0.6 μM; p<0.05). Potassium currents elicited in freshly dissociated dorsal root ganglia neurones were blocked by 31±1% (n=4; p<0.05) in the presence of 2.4 μM MiDCA1. These results show that MiDCA1 is a new presynaptic phospholipase A2 that produces neuromuscular blockade in vertebrate nerve-muscle preparations. The triphasic effects seen in mammalian preparations and the facilitatory response were probably caused mainly by the activation of sodium channels, complemented by the blockade of nerve terminal potassium channels. The inability of d-turocurarine to prevent the depolarization by MiDCA1 indicated that cholinergic nicotinic receptors were not involved in this phenomenon.
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dc.languageen
dc.publisher
dc.relationToxicon
dc.rightsfechado
dc.sourceScopus
dc.titlePharmacological And Structural Characterization Of A Novel Phospholipase A2 From Micrurus Dumerilii Carinicauda Venom
dc.typeArtículos de revistas


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